Inhibitory effect of Pimitrex disodium heptidate on the growth of CRAS-dependent A549 lung cancer cells. I do not have any relevant financial relationships to disclose. Figure 1 shows that Pimitrex-induced apoptosis evidenced by elevated expression of Clp-Caspas 9, Clp-Caspas 3, and Clp-PARP. Figure 2a reveals that Pimitrex increased the ROS level in a dose-dependent manner. The changes were accompanied by DNA fragmentation, which is represented by Figure 2b. Figure 3 indicates that increased pro-apoptotic Bax protein expression to induce loss of mitochondrial membrane potential. Figure 4 reveals that increased cellular sensors through increase of beta-galactosidase expression. Figure 5 shows that Pimitrex-induced cytotoxicity without inhibiting the RAS-REF-MEC-ERK signaling pathway but induced autophagy through activating MPK-M2 pathway. This slide indicates key outcomes of this study. Thank you very much.

Pimitrex disodium heptidate

CRAS-dependent A549 lung cancer cells

apoptosis

reactive oxygen species (ROS)

mitochondrial membrane potential