2022 World Conference on Lung Cancer (Posters)-P2.13-01. Low EV miR-30c Levels as Biomarker of Increased Tumour Autophagy and Chemoradiotherapy Resistance in Locally Advanced NSCLC

P2.13-01. Low EV miR-30c Levels as Biomarker of Increased Tumour Autophagy and Chemoradiotherapy Resistance in Locally Advanced NSCLC

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A recent study found that low levels of a specific microRNA called miR-30c in extracellular vesicles (EVs) can serve as a biomarker for increased tumor autophagy and resistance to chemoradiotherapy in locally advanced non-small cell lung cancer (NSCLC). The study, conducted by a team of researchers from various institutions in the US, Spain, Italy, Colombia, and Maryland, aimed to identify novel biomarkers that can help predict treatment response and outcomes in NSCLC patients undergoing chemoradiotherapy.<br /><br />The researchers collected blood samples from 38 patients with locally advanced NSCLC before, during, and after chemoradiotherapy. They isolated EVs and circulating tumor cells (CTCs) from the blood samples and characterized them. They also performed in vitro experiments using lung cancer cell lines to further investigate the role of miR-30c in autophagy and chemoradiation resistance.<br /><br />The findings showed that patients with low levels of EV miR-30c had increased tumor autophagy and poorer treatment response and survival outcomes. Additionally, the presence of autophagy-activated CTCs was associated with poor outcomes. The researchers suggest that EV miR-30c and autophagy-activated CTCs could be used as biomarkers to stratify and monitor NSCLC patients undergoing chemoradiotherapy and potentially guide the use of novel combination therapies with autophagy inhibitors.<br /><br />Further analysis revealed that miR-30c targets genes involved in the phosphatidylinositol-mediated signaling pathway, a regulator of autophagy. In in vitro models, the researchers found that adding a miR-30c mimic to cancer cells treated with chemoradiation reduced cellular viability and autophagy activation, while inhibiting miR-30c increased viability and autophagy activation.<br /><br />Overall, this study provides new insights into the role of EV miR-30c in tumor autophagy and chemoradiotherapy resistance in locally advanced NSCLC. The identification of this biomarker could help improve patient stratification and treatment decision-making in this patient population. Further research is warranted to validate these findings and explore the potential of combination therapies targeting autophagy.

Asset Subtitle

Diego de Miguel Perez, United States

Meta Tag

Speaker Diego de Miguel Perez, United States

Topic Tumour Biology and Biomarkers - Minimally Invasive Biomarkers

Keywords

microRNA

miR-30c

extracellular vesicles

biomarker

tumor autophagy

chemoradiotherapy resistance

non-small cell lung cancer

NSCLC

circulating tumor cells

phosphatidylinositol-mediated signaling pathway