2022 World Conference on Lung Cancer (ePosters)-EP02.02-001. Genome-wide Analysis and Dynamic Changes of Human Non-small Cell Lung Carcinoma Cells After Radiation

EP02.02-001. Genome-wide Analysis and Dynamic Changes of Human Non-small Cell Lung Carcinoma Cells After Radiation

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This study focused on understanding the effects of radiation on human non-small cell lung cancer at the genomic and time levels. The researchers irradiated A549 and H1299 lung cancer cells with a 10Gy X-ray and treated them at different time intervals. They performed whole-transcriptome sequencing and used bioinformatics methods to analyze the differences in gene expression. The STEM algorithm was used to identify genes that were differentially expressed based on time changes. The analysis of mRNA, miRNA, circRNA, and lncRNA revealed networks involved in the radiation response.<br /><br />The GO and KEGG analyses showed that the enriched functions and pathways included glucose metabolism and insulin pathways. The Ingenuity Pathway Analysis software revealed that the gene set function of lung adenocarcinoma after radiation was centered around cell cycle-related transcription factor E2F1 in the early stage, while autophagy played a role in the later stage. Toxicity pathway analysis indicated that the radiation had toxic effects on the kidney and liver.<br /><br />Cell cycle experiments showed that lung adenocarcinoma cells experienced the most significant block in the G2/M stage 24 hours after radiation. PCR and Western blot experiments confirmed the increased expression of the cell cycle-related protein CCNB1 at this time point.<br /><br />Overall, this study provides insights into the key molecules, network regulation, and disease pathways involved in the response of non-small cell lung cancer to short-range and high-dose radiation. The findings contribute to a better understanding of the effects of radiation on lung cancer and could potentially guide clinical combination treatment strategies.

Asset Subtitle

Yajing Du Yajing

Meta Tag

Speaker Yajing Du Yajing

Topic Early Stage Non-small Cell Lung Cancer - Radiotherapy

Keywords

radiation

lung cancer

genomic

time levels

gene expression

transcriptome sequencing

cell cycle

E2F1

autophagy

toxicity pathway