2022 World Conference on Lung Cancer (ePosters)-EP02.04-002. Synergy of Local Treatment with Pulsed Electric Fields and Anti-PD1 Checkpoint Blockade

EP02.04-002. Synergy of Local Treatment with Pulsed Electric Fields and Anti-PD1 Checkpoint Blockade

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A preclinical study was conducted to evaluate the combination of Pulsed Electric Fields (PEFs) with anti-PD-1 checkpoint blockade for the treatment of tumors. PEF therapy was delivered to solid EMT6 tumors in mice using the AliyaTM System. The treatment resulted in immunogenic cell death, increased T-cell infiltration, and elevated levels of HMGB1 in the serum. The combination of PEF and anti-PD-1 was found to have a synergistic effect and improved local and distant tumor control. Combinatorial therapy significantly improved survival rates and prevented the reoccurrence of tumors in a majority of the mice.<br /><br />The study also analyzed cytokine expression before and after treatment, indicating a shift to a pro-inflammatory state. The pathways affected by the cytokines included the activation of leukocytes, activation of macrophages, and infiltration of T-cells. In a follow-up study, mice that achieved total tumor clearance after PEF treatment were rechallenged with the same cell line, and the majority of the mice prevented or regressed the rechallenged tumor, suggesting a long-term adaptive tumor-specific immune response.<br /><br />In conclusion, the study demonstrates the immunogenic impact of PEF therapy and the benefits of combining it with anti-PD-1 checkpoint blockade. The combination treatment resulted in improved tumor control, increased survival rates, and the development of a long-term immune response against the tumor. These findings suggest the potential of PEF therapy as a novel treatment approach for solid tumors.

Asset Subtitle

Chiara Pastori

Meta Tag

Speaker Chiara Pastori

Topic Early Stage Non-small Cell Lung Cancer - Systemic Therapy

Keywords

preclinical study

Pulsed Electric Fields

anti-PD-1 checkpoint blockade

treatment of tumors

immunogenic cell death

T-cell infiltration

HMGB1

synergistic effect

tumor control

cytokine expression