2022 World Conference on Lung Cancer (ePosters)-EP05.01-031. Lysimachia Capillipes Capilliposide C Enhances the Radiosensitivity of Lung Cancer by Promoting ERRFI1 via Inhibiting Phosphorylation of STAT3

EP05.01-031. Lysimachia Capillipes Capilliposide C Enhances the Radiosensitivity of Lung Cancer by Promoting ERRFI1 via Inhibiting Phosphorylation of STAT3

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The study investigates the detailed mechanism of Lysimachia capillipes capilliposide C (LC-C) in increasing the expression of ErbB receptor feedback inhibitor 1 (ERRFI1), which has been shown to sensitize ionizing radiation-resistant lung cancer cells. The researchers used non-small cell lung cancer (NSCLC) cell lines and performed various assays to assess the effects of LC-C on ERRFI1 expression and radiosensitivity.<br /><br />They found that LC-C reduced the phosphorylation level of STAT3 and promoted the expression of ERRFI1 in radiation-resistant lung cancer cells. The researchers also demonstrated that a STAT3 inhibitor upregulated the mRNA and protein expressions of ERRFI1, while a STAT3 activator downregulated its expression. LC-C enhanced the transcriptional activity of the ERRFI1 promoter, which was further enhanced by the STAT3 inhibitor and reduced by the STAT3 activator. IL-6, a cytokine that activates STAT3, attenuated the effects of LC-C on ERRFI1 expression and promoter transcription activity.<br /><br />Furthermore, LC-C effectively inhibited the repair of DNA damage caused by ionizing radiation and increased the radiosensitivity of the radiation-resistant lung cancer cells. On the other hand, IL-6 attenuated these effects of LC-C on DNA damage repair and radiosensitivity.<br /><br />In conclusion, the phosphorylation of STAT3 plays a crucial role in the regulation of ERRFI1 expression and radiosensitivity in lung cancer treated with LC-C. Phosphorylated STAT3 affects ERRFI1 expression by regulating the transcriptional activity of its promoter. These findings provide insights into the mechanism of action of LC-C in enhancing the radiosensitivity of lung cancer cells and suggest its potential use as a therapeutic agent for lung cancer treatment.

Asset Subtitle

Kan Wu

Meta Tag

Speaker Kan Wu

Topic Locally Advanced Non-small Cell Lung Cancer - Chemoradiotherapy and Radiotherapy

Keywords

Lysimachia capillipes capilliposide C

ErbB receptor feedback inhibitor 1

ionizing radiation-resistant lung cancer cells

non-small cell lung cancer (NSCLC)

LC-C

phosphorylation level of STAT3

radiosensitivity

STAT3 inhibitor

STAT3 activator

IL-6