2022 World Conference on Lung Cancer (ePosters)-EP08.02-032. High Efficiency Multiplex Detection of Molecular Alterations in Supernatants of Pleural Effusion and Cerebrospinal Fluid

EP08.02-032. High Efficiency Multiplex Detection of Molecular Alterations in Supernatants of Pleural Effusion and Cerebrospinal Fluid

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The study focused on the detection of molecular alterations in the supernatants of pleural effusion (MPE) and cerebrospinal fluid (CSF) in lung cancer patients. It aimed to investigate the use of these fluids as a source of circulating free DNA (cfDNA) or RNA (cfRNA) for genetic testing. The researchers prospectively collected 30 fluid samples from lung cancer patients, consisting of 25 MPE and 5 CSF. The samples were analyzed using various techniques, including next-generation sequencing (NGS) and nCounter panels, to detect mutations, gene fusions, and copy number variations.<br /><br />The results showed that MPE and CSF can be a reliable source of tumor-derived cfDNA and cfRNA for genetic testing in lung cancer patients with central nervous system (CNS) and pleural involvement. The study found a high concordance rate (94.7%) between genetic alterations detected in the fluids and those detected in paired cell pellets or formalin-fixed, paraffin-embedded (FFPE) samples. Clinically relevant alterations were detected in 24 out of 27 fluid samples (89%), including mutations, rearrangements, and copy number gains. TP53 mutations and copy number gains were often found to co-occur with other alterations.<br /><br />The study concluded that MPE and CSF can provide valuable information for treatment decision-making in lung cancer patients with CNS and pleural involvement. The use of these fluids as a source of cfDNA and cfRNA for genetic testing could be particularly helpful in cases where additional samples are not available. The findings suggest that the implementation of this type of biological material in routine practice should be standardized to improve patient care.

Asset Subtitle

Clara Mayo de las Casas

Meta Tag

Speaker Clara Mayo de las Casas

Topic Metastatic Non-small Cell Lung Cancer - Molecular Targeted Treatments

Keywords

molecular alterations

lung cancer patients

genetic testing

fluid samples

next-generation sequencing

copy number variations

tumor-derived cfDNA

tumor-derived cfRNA

clinically relevant alterations

central nervous system involvement