2022 World Conference on Lung Cancer (ePosters)-EP08.02-119. RNA Sequencing to Characterize Pathways in EGFR-mutated Non-Small Cell Lung Cancer

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Researchers from Rush University Medical Center have conducted a study to identify potential subgroups of patients with EGFR-mutated non-small cell lung cancer (NSCLC) based on phenotypic features. The study aimed to compare patient characteristics and clinical outcomes between these subgroups. The researchers identified 287 patients with EGFR-mutated lung cancer using the Tempus Lens database. They performed unsupervised clustering analysis using RNA sequencing expression data and generated heat maps and volcano plots to visualize the results. <br /><br />From the analysis, the researchers identified two distinct subgroups of patients with EGFR-mutated lung cancer. They observed significant differences in gene expression when stratifying patients based on age (70 years old vs. <70 years old) and clinical outcome (alive vs. deceased). The heat map revealed the distribution of patients in each subgroup, with cluster P1 having 205 patients and cluster P2 having 82 patients. <br /><br />The volcano plots showed differences in gene expression between different age groups and between patients who were alive or deceased at the time of data extraction. Genes passing the significance threshold and labeled as 'red' were found to have a q-value less than 0.05. Genes labeled as 'orange' had an absolute log2 fold change of more than 2. Genes labeled as 'green' passed both the significance threshold and log2 fold change criteria.<br /><br />The researchers are hopeful that by using RNA sequencing as a tool, they can further identify differentially upregulated gene expression pathways in patients with early (stage I-II) vs. late (stage III-IV) presentations of EGFR-mutated NSCLC, as well as in patients who progress early on TKIs (6 months of therapy). This study provides valuable insights into the potential subgroups within EGFR-mutated NSCLC and highlights the importance of understanding the molecular pathways in this type of lung cancer.

Asset Subtitle

Nicole K Yun

Meta Tag

Speaker Nicole K Yun

Topic Metastatic Non-small Cell Lung Cancer - Molecular Targeted Treatments

Keywords

Rush University Medical Center

EGFR-mutated non-small cell lung cancer

phenotypic features

patient characteristics

clinical outcomes

Tempus Lens database

unsupervised clustering analysis

RNA sequencing expression data

heat maps

volcano plots