2022 World Conference on Lung Cancer (ePosters)-EP11.01-006. Setting Up an Ultra-Fast Next-Generation Sequencing Approach as a Reflex Testing at Diagnosis in Non-squamous Non-small Cell Lung Cancer

EP11.01-006. Setting Up an Ultra-Fast Next-Generation Sequencing Approach as a Reflex Testing at Diagnosis in Non-squamous Non-small Cell Lung Cancer

Back to course

Pdf Summary

A single center in Nice, France has reported their experience in setting up an ultra-fast next-generation sequencing (NGS) approach as reflex testing for non-squamous non-small cell lung cancer (NS-NSCLC) patients. The aim was to develop a strategy that allows for a rapid diagnosis in the face of increasing targetable genomic alterations and decreasing tissue material. The study used the Genexus OPA DNA RNA panel from Thermo-Fisher for ultra-fast NGS.<br /><br />The study included 259 patients with NS-NSCLC, primarily adenocarcinoma, and various types of tumor samples were analyzed, including bronchial biopsies, transthoracic biopsies, surgical specimens, and cellblocks from pleural effusion and endobronchial ultrasound with a mean turnaround time of 72 hours. The mean tumor cell percentage was 40%. The analytical validation of the Genexus OPA workflow demonstrated 100% concordance with gold standard methods.<br /><br />The study concluded that the Genexus system identified molecular targets for personalized medicine in NS-NSCLC with a rapid turnaround time. The ultra-fast NGS integration as reflex testing was deemed to be an optimal option for genomic alteration assessment at diagnosis for all stages of NS-NSCLC. This approach allows for the sensitive and specific identification of mutations, copy number variations (CNVs), and fusion variants across 50 key genes, even in tumor samples with low amounts of nucleic acids.<br /><br />The authors suggest that combining ultra-fast NGS with multiplex immunohistochemistry (IHC) approaches could provide added value in identifying the histological classification of tumors using both genomic and proteomic biomarkers simultaneously.<br /><br />In summary, the study highlights the successful implementation of an ultra-fast NGS approach as reflex testing for the rapid diagnosis of NS-NSCLC. This approach allows for the identification of genomic alterations in tumor samples with limited material and demonstrates its potential for personalized medicine in NS-NSCLC.

Asset Subtitle

Paul Hofman

Meta Tag

Speaker Paul Hofman

Topic Pathology - Genomics & Analytics

Keywords

Nice, France

ultra-fast next-generation sequencing

reflex testing

NS-NSCLC

Genexus OPA DNA RNA panel

turnaround time

molecular targets

personalized medicine

mutations

copy number variations