2022 World Conference on Lung Cancer (ePosters)-EP11.01-010. A Prospective Validation Study of Lung Cancer Gene Panel Testing Using Cytological Specimens

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In this study, researchers aimed to evaluate the feasibility of using cytological specimens for lung cancer gene panel testing. Personalized medicine for lung cancer often involves molecular-targeted drugs, but panel tests require sufficient tissue samples, which may not always be available. Therefore, the researchers sought to determine if cytological specimens, which are easier and safer to collect, could be used for gene panel testing. <br /><br />The study enrolled consecutive patients who underwent diagnostic procedures from May 2020 to January 2022. A high-sensitivity next-generation sequencing panel test was used to measure eight druggable genes associated with lung cancer. The researchers successfully extracted and analyzed nucleic acid from all 163 prospective cases, which were diagnosed with adenocarcinoma. Out of these cases, 112 (68.7%) had gene mutations, with a 98.8% concordance in companion diagnostic mutation call. The median DNA/RNA yield and integrity numbers were sufficient for panel analysis. Additionally, the gene allele ratio in cytology samples showed a high correlation (0.717) with tissue samples.<br /><br />The findings suggest that using cytological specimens for gene panel testing in lung cancer is feasible. The success rate of gene analysis was high, and the extracted nucleic acid had sufficient yield and quality. Moreover, the gene mutations detected in cytological specimens showed a high correlation with tissue specimens.<br /><br />The researchers are currently conducting a prospective verification study at multiple domestic institutions to further validate these results. The study provides promising evidence that cytological samples could potentially be used for lung cancer gene panel testing, allowing for personalized medicine approaches even when tissue samples are not available.

Asset Subtitle

Kei Morikawa

Meta Tag

Speaker Kei Morikawa

Topic Pathology - Genomics & Analytics

Keywords

cytological specimens

lung cancer gene panel testing

personalized medicine

tissue samples

feasibility

gene mutations

next-generation sequencing

correlation

nucleic acid

adenocarcinoma