2022 World Conference on Lung Cancer (ePosters)-EP16.01-008. The Effect of Cytochrome P450 Family 4 Subfamily B Member 1 (CYP4B1) on Lung Adenocarcinoma (LUAD) Immune Microenvironment

EP16.01-008. The Effect of Cytochrome P450 Family 4 Subfamily B Member 1 (CYP4B1) on Lung Adenocarcinoma (LUAD) Immune Microenvironment

Back to course

Pdf Summary

Researchers from the Jordan University of Science and Technology have conducted a study on Cytochrome P450 Family 4 Subfamily B Member 1 (CYP4B1) and its implications on the immune microenvironment of lung adenocarcinoma (LUAD). CYP4B1 is predominantly expressed in the lungs and plays a role in immune regulation. The study aimed to understand the immune landscape of CYP4B1 and its association with immune inhibitory components.<br /><br />The researchers analyzed gene expression profiles from the TCGA-LAUD dataset, which included 567 samples. They used the TIMER 2.0 tool to analyze immune inhibitory cell infiltrates. They also measured TMB and MSI scores using the cBioPortal tool. <br /><br />The results showed that CYP4B1 was differentially expressed in LUAD tissues compared to normal liver tissues. High CYP4B1 expression was associated with favorable overall survival in LUAD patients. CYP4B1 expression was negatively correlated with myeloid-derived suppressor cells (MDSC) infiltration but positively correlated with macrophage M2 cells. Survival analysis based on CYP4B1 expression and MDSC levels revealed a worse overall survival group identified by low CYP4B1 expression and high MDSC levels.<br /><br />Additionally, CYP4B1 expression was directly correlated with immune inhibitory markers such as PD-1, PD-L1, VSIR, and LAG-3. It was inversely associated with TMB count and had an inverse association with the MSI MANTIS score.<br /><br />The researchers performed gene ontology analysis and found that genes positively co-expressed with CYP4B1 were mainly located in the plasma membrane and participated in biological processes related to testosterone stimuli and actin filament reorganization. The top enriched KEGG pathways linked with CYP4B1 expression were nitrogen metabolism, proximal tubule bicarbonate reclamation, and GnRH secretion.<br /><br />Based on their findings, the researchers concluded that CYP4B1 could serve as a useful marker of immunosuppression in LUAD patients and could potentially predict the response to immunotherapy. This study provides valuable insights into the role of CYP4B1 in the immune microenvironment of LUAD.

Asset Subtitle

Leen Al-Kraimeen

Meta Tag

Speaker Leen Al-Kraimeen

Topic Tumour Biology and Biomarkers - Immune Biology & Immunotherapy

Keywords

CYP4B1

immune microenvironment

LUAD

gene expression profiles

overall survival

MDSC

PD-1

PD-L1

KEGG pathways

immunotherapy