2022 World Conference on Lung Cancer (ePosters)-EP16.02-001. Integration of Primer Exchange Reaction and DNAzyme for Electrochemical Detection of PD-L1-Expressing Exosomes in Lung Cancer

EP16.02-001. Integration of Primer Exchange Reaction and DNAzyme for Electrochemical Detection of PD-L1-Expressing Exosomes in Lung Cancer

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This study presents a novel electrochemical method for the detection of PD-L1-expressing exosomes, which are considered tumor-specific biomarkers in lung cancer. The method combines primer exchange reaction (PER) with a DNAzyme called CRISPR/Cas12a, offering high sensitivity and specificity. The process involves capturing the target exosomes using PD-L1 antibodies attached to magnetic beads, followed by the recruitment of a cholesterol-modified hairpin template. After magnetic separation, the hairpin template acts as a catalytic component in the PER, triggering the synthesis of single-stranded DNA.<br /><br />The developed method demonstrates the ability to detect target exosomes within a wide linear range, ranging from 103 particles/mL to 108 particles/mL, with a detection limit of 672 particles/mL. It was also successfully applied to determine the level of PD-L1-expressing exosomes in clinical samples from patients with non-small cell lung cancer. The electrochemical analysis showed enhanced peak currents in samples from patients compared to healthy donors, indicating a positive correlation between the peak current enhancement and lung cancer progression and prognosis.<br /><br />The study concludes that the proposed method can effectively distinguish lung cancer patients from healthy individuals and can provide insights into the progression of the disease. This suggests the potential use of the method for screening lung cancer patients and monitoring the progression of the disease.<br /><br />In summary, the integration of the PER and CRISPR/Cas12a-based DNAzyme in an electrochemical platform provides a sensitive and specific approach for the detection of PD-L1-expressing exosomes in lung cancer. The method's successful application in clinical samples highlights its potential utility in lung cancer diagnosis and disease monitoring.

Asset Subtitle

Bing Bo

Meta Tag

Speaker Bing Bo

Topic Tumour Biology and Biomarkers - Minimally Invasive Biomarkers

Keywords

electrochemical method

detection

PD-L1-expressing exosomes

tumor-specific biomarkers

lung cancer

primer exchange reaction

DNAzyme

CRISPR/Cas12a

sensitivity

specificity