2022 World Conference on Lung Cancer (ePosters)-EP16.02-019. Metabolic Changes in Sputum and Exhaled Breath Condensate of Non-small Cell Lung Cancer Patients after Surgical Resection

EP16.02-019. Metabolic Changes in Sputum and Exhaled Breath Condensate of Non-small Cell Lung Cancer Patients after Surgical Resection

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This study explored the metabolic changes in sputum and exhaled breath condensate (EBC) of non-small cell lung cancer (NSCLC) patients before and after surgical resection (SR) as a potential noninvasive diagnostic tool. The researchers collected samples of EBC and cytologically confirmed sputum from 35 patients pre- and post-SR. They used nuclear magnetic resonance (NMR) and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) to analyze the metabolic profile of the samples. <br /><br />The results showed significant metabolic changes in both sputum and EBC post-SR. Specifically, 26 metabolites, including lipids and other chemical metabolites, showed significant changes. 18 metabolites were found to be upregulated, while 8 were downregulated. The concentration of upregulated and downregulated metabolites showed a median fold change of 10 and 8, respectively, for LC-QTOF-MS analysis. The fold change in concentration for the upregulated and downregulated metabolites in NMR analysis was 0.04 and 0.27, respectively. <br /><br />The researchers observed a consistent pattern of dysregulation in sputum post-SR compared to pre-surgery, suggesting that sputum analysis may be more robust and clinically significant than EBC analysis. They also found that sputum levels of glucose, adenosine monophosphate, and N1, N12-diacetylspermine were significantly elevated post-SR. This could potentially indicate a pre- or early malignant process and serve as a promising noninvasive tool for early NSCLC diagnosis. <br /><br />Overall, this study highlights the metabolic changes in sputum and EBC of NSCLC patients after SR, suggesting that analyzing these metabolites could potentially be used as a noninvasive diagnostic method for early detection of NSCLC. Further research is needed to validate these findings and explore their clinical implications.

Asset Subtitle

Naseer Ahmed

Meta Tag

Speaker Naseer Ahmed

Topic Tumour Biology and Biomarkers - Minimally Invasive Biomarkers

Keywords

metabolic changes

sputum

exhaled breath condensate

NSCLC

surgical resection

noninvasive diagnostic tool

lipids

upregulated metabolites

downregulated metabolites

early NSCLC diagnosis