2022 World Conference on Lung Cancer (ePosters)-EP16.03-041. Single Cell RNA Sequencing Reveals Phenotypic Predispositions to Developing Lung Cancer in Never-Smokers

EP16.03-041. Single Cell RNA Sequencing Reveals Phenotypic Predispositions to Developing Lung Cancer in Never-Smokers

Pdf Summary

A study conducted by researchers at The Garvan Institute of Medical Research and The University of Sydney aimed to investigate the genetic variability in gene expression to understand the predispositions to developing lung cancer (LC) in never-smokers. LC in never-smokers is a growing problem, and impaired cellular repair of DNA damage and response to chronic inflammation are potential mechanisms of the disease.<br /><br />The researchers compared peripheral immune cells from 19 never-smoking LC patients with age-matched never-smoking controls. They performed differential expression and expression quantitative trait loci (eQTL) analyses. The results showed that the cell proportions differed between the two groups, indicating a phenotypic difference. The eQTL analysis revealed that SNPs in genes on chromosome 6 responsible for antigen (Ag) processing tend to result in reduced gene expression.<br /><br />The study findings suggest that differences in the Ag processing pathway may be a predisposing factor in developing LC in individuals who have never smoked. This information contributes to a better understanding of the genetic factors involved in the development of LC in never-smokers.<br /><br />Overall, the study utilized single-cell sequencing methods to demonstrate that LC patients who have never smoked have different cell proportions compared to controls. Additionally, the eQTL analysis identified specific genes on chromosome 6 that are associated with Ag processing and reduced gene expression. These findings shed light on the potential genetic predispositions to developing LC in never-smokers and may help in the identification of new targeted therapies or preventive measures for this population.

Asset Subtitle

Venessa Chin

Meta Tag

Speaker Venessa Chin

Topic Tumour Biology and Biomarkers - Molecular Profiling and Targeted Therapies

Keywords

genetic variability

gene expression

lung cancer

never-smokers

cellular repair

DNA damage

chronic inflammation

peripheral immune cells

differential expression

expression quantitative trait loci