2022 World Conference on Lung Cancer (ePosters)-EP16.04-001. The Role of Stromal PDGFR-activation in NSCLC

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The study focuses on the role of stromal platelet-derived growth factor receptor beta (PDGFRb) activation in non-small cell lung cancer (NSCLC). The researchers aimed to develop an in situ assay to detect activated PDGFRb and investigate its clinical relevance and prognostic potential for NSCLC patients. They used the proximity ligation assay (PLA) with UnFold probes to target active PDGFRb and adapted the signal-detection to the tyramide signal amplification method. They also performed other staining techniques to differentiate between stromal and tumor areas. <br /><br />The results showed that the distribution of activated PDGFRb correlated with general protein expression of PDGFRb as investigated by immunohistochemistry (IHC) and there was no difference in distribution between different histologies of NSCLC. However, the activation status of PDGFRb did not show significant associations with survival outcome in NSCLC. <br /><br />Further analysis revealed that genes associated with PDGF/PDGFR binding were upregulated with an increased PLA score of PDGFRb activation. These upregulated genes were also associated with changes in extracellular matrix structure and composition, suggesting a potential role for PDGF signaling in matrix remodeling. <br /><br />In conclusion, the study successfully developed a method to detect active PDGFRb in NSCLC tissue samples. However, the activation status of PDGFRb did not have a strong association with survival outcome in NSCLC. The findings suggest that PDGFRb activation may play a role in extracellular matrix changes in NSCLC.

Asset Subtitle

Amanda Lindberg

Meta Tag

Speaker Amanda Lindberg

Topic Tumour Biology and Biomarkers - Tumour Biology & Preclinical Studies

Keywords

stromal platelet-derived growth factor receptor beta

PDGFRb activation

non-small cell lung cancer

in situ assay

clinical relevance

proximity ligation assay

tyramide signal amplification

immunohistochemistry

survival outcome

extracellular matrix structure