2022 World Conference on Lung Cancer (ePosters)-EP16.04-004. Impact of Gender on Cellular Response to Cigarette Smoke Extract

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The study aimed to understand the differential responses between males and females to cigarette smoke extract (CSE) exposure in relation to lung cancer (LC) development. The researchers discovered that CSE can impact cellular proliferation, with female cell lines being more susceptible. They also identified differential expression of selected markers following CSE exposure and between cell lines. Eleven differentially expressed genes (DEGs) were validated as potential mediators of CSE-exposure and sex differences in LC pathogenesis. Changes in BLM and MAPK12 were confirmed by in silico data and were identified as markers of interest. <br /><br />The study was conducted by researchers from Karolinska Institutet and Trinity College Dublin. The research showed that exposure to CSE resulted in dose-dependent decreases in cellular proliferation over time, with a higher susceptibility observed in female cell lines. The study also compared mRNA expression based on CSE treatment and sex, leading to the identification of 11 DEGs that were further validated as potential biomarkers for LC.<br /><br />The researchers found upregulation of TLR4 in HBEC3, ATM, and MAPK12 in HBEC4 following CSE exposure. They also observed distinct upregulation of selected DEGs between cell lines. The study further confirmed increased MAPK12 in smokers and increased BLM in males using patient sample data.<br /><br />The study suggests future directions for research, including investigating changes at the protein level, examining gene expression in clinical samples of smokers and non-smokers, determining the role of metabolic enzymes, conducting mechanistic studies, and using more physiologically relevant models such as 3D cultures.<br /><br />In conclusion, the study highlights the differential response to CSE exposure between males and females and identifies potential biomarkers for LC pathogenesis. Further research is needed to understand the underlying mechanisms and to develop targeted detection, diagnosis, and treatment strategies for lung cancer.

Asset Subtitle

Anne-Marie Baird

Meta Tag

Speaker Anne-Marie Baird

Topic Tumour Biology and Biomarkers - Tumour Biology & Preclinical Studies

Keywords

cigarette smoke extract exposure

lung cancer development

cellular proliferation

female cell lines

differential expression

selected markers

differentially expressed genes

CSE-exposure

sex differences

LC pathogenesis