2022 World Conference on Lung Cancer (ePosters)-EP16.04-010. Histological and Genetic Parental Tumor Characteristics are Conserved in Patient-derived Non-small Cell Lung Cancer Organoids

EP16.04-010. Histological and Genetic Parental Tumor Characteristics are Conserved in Patient-derived Non-small Cell Lung Cancer Organoids

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This study aimed to establish patient-derived non-small cell lung cancer (NSCLC) organoids as representative in-vitro models for precision medicine. Surgically resected NSCLC tissue specimens were collected and processed, and cell suspensions were mixed with Matrigel and cultured in growth medium. Histology, immunohistochemistry, and genetic validation were performed to confirm that the organoids maintained the morphological characteristics and genetic alterations of the parental tumor. Among the 34 resected NSCLC samples, 17 primary organoid cultures were successfully established and expanded. The organoids showed similar characteristics to the parental tumor, including different types of NSCLC morphology. Traceable genetic alterations were detected in six parental tumors, and two corresponding organoid lines retained this mutational profile. These findings support the limited establishment rates of genetically validated NSCLC organoids. By comparing different medium conditions, a standard medium supplemented with A83-01 and Noggin resulted in the highest establishment and growth rates for organoids from adenocarcinoma and squamous cell carcinoma tissues. <br /><br />Overall, this study demonstrates the successful establishment of patient-derived NSCLC organoids that maintain both histological and genetic characteristics of the parental tumor. This provides a promising platform for further investigations and personalized cancer treatments. However, the establishment rates of these organoids remain a challenge for broader clinical applications. The study was funded by the Stiftung für angewandte Krebsforschung (SAKF), Iten-Kohaut-Stiftung, and Kurt und Senta Herrmann-Stiftung. <br /><br />Reference:<br />Dijkstra KK, Monkhorst K, Schipper LJ, et al. Challenges in Establishing Pure Lung Cancer Organoids Limit Their Utility for Personalized Medicine. Cell Rep. 2020;31:107588.

Asset Subtitle

Isabelle Opitz

Meta Tag

Speaker Isabelle Opitz

Topic Tumour Biology and Biomarkers - Tumour Biology & Preclinical Studies

Keywords

patient-derived non-small cell lung cancer

NSCLC organoids

in-vitro models

precision medicine

surgically resected NSCLC tissue

Matrigel

growth medium

genetic validation

morphological characteristics

parental tumor