2022 World Conference on Lung Cancer (ePosters)-EP16.04-017. Lineage Switching and Tumor Microenvironment Roles of Tumor Suppressors in Autochthonous Lung Cancer Models

EP16.04-017. Lineage Switching and Tumor Microenvironment Roles of Tumor Suppressors in Autochthonous Lung Cancer Models

Back to course

Pdf Summary

This paper discusses the roles of the Trp53 and Pten genes in lung cancer development and the tumor microenvironment (TME). The study utilizes a mouse model to investigate the effects of these genes on lung cancer differentiation, metastasis, and TME composition.<br /><br />The results show that loss of Pten or Trp53 leads to increased tumor formation, decreased survival, and the formation of more adenocarcinoma tumors than squamous lung tumors. Additionally, the study finds that squamous tumors recruit higher levels of tumor-promoting immune infiltrates, such as inflammatory monocytes and macrophages. Conversely, immune populations normally protective against cancer progression, such as dendritic cells, are less abundant in squamous models.<br /><br />The researchers also establish a metastatic lung squamous cell (LUSC) model that can be grown in fully immune competent mice. This model is used to evaluate combinatorial immune checkpoint inhibitor (ICI) therapies and identify potential biomarkers predictive of response to α-PD1 and α-CTLA4 treatment.<br /><br />The paper provides details on the methods used in the study, including tracheal instillation of lentivirus to induce lung carcinomas in mice and the creation of a C57BL/6 compatible metastatic LUSC cell line.<br /><br />Overall, this study highlights the importance of the Trp53 and Pten genes in lung cancer development and the distinct roles they play in different lung cancer subtypes. The findings also shed light on the impact of these genes on the tumor microenvironment and suggest potential biomarkers for predicting response to immune checkpoint inhibitor therapies in LUSC.

Asset Subtitle

Nisitha Sengottuvel

Meta Tag

Speaker Nisitha Sengottuvel

Topic Tumour Biology and Biomarkers - Tumour Biology & Preclinical Studies

Keywords

Trp53

Pten

lung cancer development

tumor microenvironment

adenocarcinoma tumors

squamous lung tumors

immune infiltrates

metastatic LUSC model

immune checkpoint inhibitor therapies

biomarkers predictive of response