2022 World Conference on Lung Cancer (ePosters)-EP16.04-031. Connexin 43 Overexpression Induces Angiogenesis in Vitro Following Phosphorylation at Ser279 in its C Terminus

EP16.04-031. Connexin 43 Overexpression Induces Angiogenesis in Vitro Following Phosphorylation at Ser279 in its C Terminus

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The role of connexin 43 (Cx43) in angiogenesis, the formation of new blood vessels, and its signaling pathways were investigated in this study. Researchers found that overexpression of Cx43 in human pulmonary microvascular endothelial cells increased the expression of several proteins involved in angiogenesis, including zonula occludens-1 (ZO-1), E-cadherin, β-catenin, von Willebrand factor (vWF), and plasminogen activator inhibitor-1 (PAI-1). Additionally, Cx43 overexpression led to an increase in the phosphorylation of Ser279, a specific site on Cx43's C-terminus, as well as increased cell proliferation, migration, and blood vessel formation. On the other hand, when Cx43 expression was knocked down, the levels of these proteins and phosphorylation of Ser279 were decreased, resulting in reduced cell proliferation, migration, and angiogenesis. Furthermore, membrane Cx43 content was also affected by Cx43 overexpression or knockdown, with an increase and decrease, respectively. These findings suggest that Cx43 overexpression can induce angiogenesis in vitro by promoting cell proliferation and migration, as well as activating ZO-1, E-cadherin, β-catenin, vWF, and PAI-1. This activation may be driven by phosphorylation of Ser279 on the C-terminus of Cx43. Overall, this study provides insights into the role of Cx43 in lung cancer angiogenesis and highlights potential signaling pathways that could be targeted for therapeutic interventions against tumor growth and metastasis.

Asset Subtitle

Zizi Zhou

Meta Tag

Speaker Zizi Zhou

Topic Tumour Biology and Biomarkers - Tumour Biology & Preclinical Studies

Keywords

connexin 43

angiogenesis

blood vessels

signaling pathways

pulmonary microvascular endothelial cells

phosphorylation

cell proliferation

cell migration

blood vessel formation

lung cancer