2023 World Conference on Lung Cancer (Posters)-EP02.04. Integrative Single Cell RNA Sequencing Reveals New Insight in Lung Cancer Microenvironment

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This study focused on using integrative single cell RNA sequencing to gain new insights into the lung cancer microenvironment. Previous studies have examined the tumor microenvironment of lung cancer, but the use of single cell RNA sequencing has allowed for more extensive and specific analysis of cell types within the microenvironment. However, there have been inconsistencies in the results due to variations in target cells and experimental techniques. Thus, the researchers aimed to conduct integrative analysis of multiple single cell RNA sequencing datasets to provide new perspectives on the lung cancer microenvironment.<br /><br />The methods involved using the Seurat package and CCA method to analyze multiple accessible single cell RNA sequencing datasets from the Gene Expression Omnibus. The researchers identified composition ratio changes and differentially expressed genes of each defined cell cluster across different conditions. They also conducted Geneset Enrichment Analysis (GSEA), pseudotime analysis, and analyzed cell-to-cell interactions using the CellChat package.<br /><br />The results showed differences in the population composition of immune cells between different conditions, specifically in resident lung macrophages and blood-originated myeloid cells. However, the overall pattern of T and B cells was similar. Significant interactions were observed between immune cells of the tumor and resident lung macrophages, suggesting the important role of resident macrophages in the tumor microenvironment.<br /><br />In conclusion, this study analyzed multiple single cell RNA sequencing datasets to gain insights into the lung cancer microenvironment. The findings highlighted differences in the population composition of immune cells in the normal lung microenvironment and indicated interactive relationships between normal lung structural cells and tumor-infiltrated immune cells. Overall, this integrative approach provides new perspectives and understanding of the lung cancer microenvironment.

Asset Subtitle

Duk Ki Kim

Meta Tag

Speaker Duk Ki Kim

Topic Tumor Biology: Preclinical Biology - Tumor Immunity

Keywords

integrative single cell RNA sequencing

lung cancer microenvironment

tumor microenvironment

single cell RNA sequencing

cell types

resident lung macrophages

blood-originated myeloid cells

T cells

B cells

immune cells