2023 World Conference on Lung Cancer (Posters)-EP02.04. Xenotransplanted Lung Tumors Display Variable Pleomorphic Patterns in Immunosuppressed BALB/c Mice

EP02.04. Xenotransplanted Lung Tumors Display Variable Pleomorphic Patterns in Immunosuppressed BALB/c Mice - PDF(Abstract)

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This study aims to evaluate a protocol for xenotransplanting lung tumors into immunosuppressed mice, specifically BALB/c mice. The researchers inject A549 cell lines and patient-derived non-small cell lung adenocarcinoma primary cultures intradermally into the mice and observe their behavior and histopathological changes. They use a combination of cyclosporin injection and oral gavage of ketoconazole to induce immunosuppression in the mice.<br /><br />The mice exhibited lethargy, with the A549 injected mice showing a moribund phenotype within two weeks and being dissected, while the patient-derived tumors lasted for over 30 days before dissection. After dissecting the organs, the researchers found extensive tumor-like cells in various organs, including the lungs, liver, and limbs. The A549 injected mice showed more tumor growth compared to the patient-derived tumors. Fluorescence microscopy using labeled antibodies confirmed the presence of these pleomorphic cells in the lungs and other tissues. However, immunohistochemistry-based confirmation for TTF1 and TP53 did not show positive detection.<br /><br />The results suggest that xenotransplanting tumors into immunosuppressed BALB/c mice is a potential protocol for simulating tumor metastasis in mice. This protocol could be used for therapeutic guidance and may be a cost-effective alternative to using athymic mice in low-resource settings.<br /><br />Further investigation is needed to understand whether the modification of tumor morphology and the absence of TTF1 and TP53 expression are due to the interaction between tumor cells and the mouse environment or the early stage of tumor development.<br /><br />Overall, this study highlights the potential of using xenotransplantation in immunosuppressed BALB/c mice as a simple and affordable method for studying tumor biology and guiding therapeutic interventions.

Asset Subtitle

Maria Isabel Idolor

Meta Tag

Speaker Maria Isabel Idolor

Topic Tumor Biology: Preclinical Biology - Tumor Immunity

Keywords

xenotransplantation

lung tumors

immunosuppressed mice

BALB/c mice

A549 cell lines

non-small cell lung adenocarcinoma

immunosuppression

tumor metastasis

tumor biology

therapeutic interventions