2023 World Conference on Lung Cancer (Posters)-EP03.03. Immunological Profiling of PD-1-Treated Murine Model Reveals Meyloid-Enriched Phenotypes

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This presentation at the WCLC 2023 conference discusses the immunological effects of PD-1 antibody treatment on a murine model of lung cancer. PD-1 antibody is a first-line treatment for lung cancer and has been shown to improve overall survival. The mechanism of action involves activating the tumoricidal role of T cells by inhibiting the PD-1 immune checkpoint. However, the effects of PD-1 antibody extend beyond T lymphocytes. <br /><br />In this study, the researchers established murine models with or without tumor xenografts to examine the impact of PD-1 on the immune environment. They used Lewis lung cancer cells to generate the cell-line derived xenografts. PD-1 therapy was initiated after the tumor became palpable. The mice were divided into three groups: control group, PD-1-treated group, and PD-1-treated plus CDX group. PD-1 monoclonal antibody was administered for 2 weeks at a concentration of 3mg/kg every 2 days. <br /><br />After the treatment period, blood, spleen, lung, bone marrow, and tumor tissues were harvested for analysis. Single-cell suspensions were obtained and analyzed using CyTOF. The results showed that CD45 immune cells accounted for more than 50% of all cell subsets. However, there were no significant variations in the proportion of cell subsets or gene expression levels between the different groups. <br /><br />Interestingly, the researchers found that certain myeloid cell subsets, such as CD11b, CD24, and CD44, were significantly enriched in the overall cell populations. This suggests that these myeloid cell subsets may be associated with the response to PD-1 therapy. <br /><br />The authors conclude that myeloid derived DC, macrophages, and inhibitory B cells are the most variable cell subsets in the tumor microenvironment. They suggest that further research should focus on other immune components that affect anti-tumor immunity. Additionally, the use of a humanized mouse model may help simulate the immune environment of patients and provide more opportunities for translational medicine.

Asset Subtitle

Xiaoshen Zhang

Meta Tag

Speaker Xiaoshen Zhang

Topic Tumor Biology: Translational Biology - IO

Keywords

PD-1 antibody treatment

immunological effects

murine model

lung cancer

T cells

tumor xenografts

monoclonal antibody

myeloid cell subsets

tumor microenvironment

translational medicine