2023 World Conference on Lung Cancer (Posters)-EP03.04. Detection of Possible Targets in Aggressive Lung Cancer Using Molecular Abundances and Catalytic Action of Serine Hydrolases

EP03.04. Detection of Possible Targets in Aggressive Lung Cancer Using Molecular Abundances and Catalytic Action of Serine Hydrolases - PDF(Abstract)

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This document discusses a study that aims to detect possible targets in aggressive lung cancer using molecular abundances and the catalytic action of serine hydrolases. The researchers utilized a SWATH/DIA-MS workflow and a depletion-dependent ABPP method to simultaneously determine the catalytically active enzymes and their total molecular abundances in lung biopsies from patients with different types of lung adenocarcinoma. <br /><br />The results showed that the molecular signatures of the activity profiles of certain enzymes and contextual tissue proteins can discriminate aggressive tumors at the time of diagnosis. The researchers also observed enhanced detection of S-palmitoylated proteins, which are functionally linked to metabolic serine hydrolases involved in lipoprotein depalmitoylation and fatty acid metabolism. <br /><br />Furthermore, the study found a statistically significant increase in monounsaturated palmitoleic acid in aggressive tumors compared to less-aggressive tumors. This increase is believed to be related to the enhanced activity of the rate-limiting enzyme Stearoyl-CoA desaturase 1 (SCD1) in lung cancer cells. <br /><br />The researchers suggest that these findings could be useful for future therapeutic strategies in lung cancer, as inhibitors of serine lipases and SCD1 have shown promise in other RAS-mutant carcinomas. These metabolic vulnerabilities detected in aggressive lung adenocarcinoma could potentially be targeted to develop new treatments for the disease. <br /><br />In conclusion, this study highlights the importance of understanding the molecular abundances and catalytic action of enzymes in aggressive lung cancer and identifies potential targets for therapeutic intervention. The findings contribute to the field of tumor biology and provide insights into the biomarkers and proteomics of lung adenocarcinoma.

Asset Subtitle

Sven Hillinger

Meta Tag

Speaker Sven Hillinger

Topic Tumor Biology: Translational Biology - Novel Targets

Keywords

lung cancer

molecular abundances

serine hydrolases

SWATH/DIA-MS workflow

lung biopsies

lung adenocarcinoma

fatty acid metabolism

Stearoyl-CoA desaturase 1

therapeutic strategies

metabolic vulnerabilities