2023 World Conference on Lung Cancer (Posters)-EP06.03. Characteristics of Insufficient Samples for EGFR Mutation Testing at the Lung Center of the Philippines

EP06.03. Characteristics of Insufficient Samples for EGFR Mutation Testing at the Lung Center of the Philippines - PDF(Abstract)

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This study aimed to describe the characteristics of samples that were considered "invalid" for EGFR mutation testing at the Lung Center of the Philippines. EGFR mutation testing is important for determining if patients with non-small cell lung cancer would benefit from targeted therapy. The study focused on the sample source and tumor content of the insufficient samples.<br /><br />The institution had a criterion that the sample must have at least 10% tumor content for analysis. However, some samples with less than 10% tumor content were still analyzed as requested by the physician. The samples used in the study were formalin-fixed paraffin-embedded blocks, and DNA was extracted using specific kits.<br /><br />The results showed that out of 1029 samples analyzed in 2018 and 2019, 25 samples were considered invalid even after repeated extraction and testing. The flags associated with invalidity indicated poor amplifiability of the DNA template. Among these samples, 8 were cytology, 8 were non-surgical biopsy, and 9 were surgical biopsy. In terms of tumor content, 5 samples had less than 10%, 12 had 10-50%, and 8 had greater than 50% tumor. Histological samples had a higher frequency of being insufficient compared to cytological samples. The number of failed tests did not differ greatly between non-surgical and surgical samples, and most insufficient samples had 10-50% tumor.<br /><br />The study concludes that further investigation is needed to evaluate other parameters for characterizing insufficient samples. This information is important for ensuring accurate EGFR mutation testing and determining the appropriate therapy for patients with non-small cell lung cancer.

Asset Subtitle

Francisco Heralde, III

Meta Tag

Speaker Francisco Heralde, III

Topic Pathology & Biomarkers: Genetic Biomarkers

Keywords

EGFR mutation testing

invalid samples

Lung Center of the Philippines

non-small cell lung cancer

targeted therapy

tumor content

DNA extraction

insufficient samples

histological samples

investigation