2023 World Conference on Lung Cancer (Posters)-EP06.06. Comparison of RICTOR FISH and Rictor Immunohistochemistry in Lung Squamous Cell Carcinoma

EP06.06. Comparison of RICTOR FISH and Rictor Immunohistochemistry in Lung Squamous Cell Carcinoma - PDF(Abstract)

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This study aims to compare the use of RICTOR FISH (fluorescence in situ hybridization) and Rictor immunohistochemistry as methods for detecting RICTOR amplification in lung squamous cell carcinoma (SQCC). RICTOR is a scaffold protein involved in cell survival and cytoskeletal reorganization, and its amplification has been observed in various cancer types, including 15% of lung SQCC. Targeted therapy for patients with RICTOR amplification is an important consideration. <br /><br />The researchers performed FISH for RICTOR gene amplification and immunohistochemistry for Rictor protein expression in 46 cases of lung SQCC. They found RICTOR amplification in 20% of the cases and Rictor membrane staining in 17% of the cases. Rictor membrane staining showed high specificity (95%) and sensitivity (77%) in predicting RICTOR amplification. <br /><br />The analysis of a public data set of 511 lung SQCC cases also showed similar results, with RICTOR amplification in 16% of the cases. RICTOR copy numbers correlated with Rictor mRNA and protein expression. <br /><br />Based on the correlation between RICTOR FISH and Rictor immunohistochemistry, as well as the correlation between RICTOR copy numbers and Rictor mRNA and protein expression, the researchers suggest that Rictor immunohistochemistry can potentially be used as a surrogate marker to select lung SQCC cases with RICTOR amplification. These patients may benefit from mTORC2 inhibitor therapy. <br /><br />This study indicates that Rictor immunohistochemistry can be a useful tool in identifying lung SQCC cases with RICTOR amplification, allowing for more targeted therapy options.

Asset Subtitle

Andras Khoor

Meta Tag

Speaker Andras Khoor

Topic Pathology & Biomarkers: Prognostic Biomarkers

Keywords

RICTOR FISH

fluorescence in situ hybridization

Rictor immunohistochemistry

detecting RICTOR amplification

lung squamous cell carcinoma

scaffold protein

cell survival

cytoskeletal reorganization

targeted therapy

mTORC2 inhibitor therapy