2023 World Conference on Lung Cancer (Posters)-EP12.03. A Retrospective Analysis on the Lung Cancer Specimens for Multi-CDx System in Keio University Hospital

EP12.03. A Retrospective Analysis on the Lung Cancer Specimens for Multi-CDx System in Keio University Hospital - PDF(Slides)

Back to course

Pdf Summary

A retrospective analysis was conducted at Keio University Hospital to evaluate the performance of the Oncomine Dx Target Test Multi CDx System (ODxTT) as a companion diagnostic tool for non-small cell lung cancer (NSCLC). The study aimed to identify cases where driver gene mutations were not detected by ODxTT despite being positive in other tests. A total of 90 NSCLC specimens were analyzed using both ODxTT and the EGFR clamp method. The results showed that EGFR clamp test results were obtained for all 90 specimens, while ODxTT yielded results for 85 DNA samples and 89 RNA samples. The success rate of ODxTT was higher for nonoperative specimens compared to surgical specimens. The study also identified two cases where there were discrepancies between the EGFR clamp method and ODxTT in detecting EGFR gene mutations. These rare variants were successfully detected by the EGFR clamp method but not by ODxTT. The study concludes that while ODxTT is generally effective, there are rare EGFR gene mutations that it may not detect, suggesting the need for additional genetic testing in cases where ODxTT yields negative results. The findings highlight the importance of careful assessment and consideration of driver gene mutations beyond the use of ODxTT alone.

Asset Subtitle

Ayaka Saito

Meta Tag

Speaker Ayaka Saito

Topic Metastatic NSCLC: Targeted Therapy - Other

Keywords

retrospective analysis

Keio University Hospital

Oncomine Dx Target Test Multi CDx System

companion diagnostic tool

non-small cell lung cancer

NSCLC

driver gene mutations

EGFR clamp method

DNA samples

RNA samples