2023 World Conference on Lung Cancer (Posters)-P1.09. DCBLD2 Impacts Tumor Immune-Vascular Microenvironment of Lung Cancer by Inducing Pro-angiogenic Macrophages

P1.09. DCBLD2 Impacts Tumor Immune-Vascular Microenvironment of Lung Cancer by Inducing Pro-angiogenic Macrophages - PDF(Slides)

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A new study published in the journal Cancer Science explores the role of DCBLD2 in the tumor immune-vascular microenvironment (TME) of lung cancer. The researchers from Southern Medical University in Guangzhou, China, found that DCBLD2 promotes angiogenesis and induces the formation of pro-angiogenic macrophages, which in turn contribute to the development of an immunosuppressive TME.<br /><br />The study involved both in vivo and in vitro experiments to investigate the impact of DCBLD2 on tumor growth, prognosis, and the TME. The researchers utilized CD31 staining of tumor tissue to confirm DCBLD2's role in promoting angiogenesis. They also co-cultured lung cancer cells with THP1 cells to demonstrate how tumor cells educate macrophages.<br /><br />The researchers analyzed single-cell RNA sequencing data to study the influence of DCBLD2 on the TME. The results were further validated through FACS assay, immunohistochemistry, and immunofluorescence staining. The findings revealed that DCBLD2-highly expressed lung cancer cells play a key role in domesticating pro-angiogenic macrophages, known as ISG15 pro-angiogenic TAMs. These macrophages contribute to the development of an immunosuppressive TME, characterized by increased CD31 vessel distribution, infiltration of TAMs, and reduced CD8 T cells.<br /><br />Furthermore, the study highlights the clinical significance of DCBLD2 and ISG15 in lung cancer. High levels of DCBLD2 expression were associated with poor prognosis and angiogenesis in lung cancer patients. The presence of ISG15 pro-angiogenic TAMs correlated with a worse prognosis in patients with lung cancer who received immunotherapy.<br /><br />Overall, this study provides valuable insights into the role of DCBLD2 in the tumor immune-vascular microenvironment of lung cancer. The findings indicate that targeting DCBLD2 and pro-angiogenic macrophages may hold promise as a therapeutic strategy for lung cancer patients.

Asset Subtitle

Bai Xue

Meta Tag

Speaker Bai Xue

Topic Tumor Biology: Translational Biology - IO

Keywords

DCBLD2

tumor immune-vascular microenvironment

lung cancer

angiogenesis

pro-angiogenic macrophages

immunosuppressive TME

CD31 staining

THP1 cells

single-cell RNA sequencing

ISG15 pro-angiogenic TAMs