2023 World Conference on Lung Cancer (Posters)-P1.11. Application of the CRISPR/Cas9-based Gene Editing Technology for the Treatment of NUT Carcinoma

P1.11. Application of the CRISPR/Cas9-based Gene Editing Technology for the Treatment of NUT Carcinoma - PDF(Slides)

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This document contains a variety of information related to NUT Carcinoma, a type of primary lung cancer. It includes data on RNA expression levels of several genes under different conditions, such as wild type (WT) and knockout (KO) of the NUTM1 gene. The expression levels are measured in transcripts per million (TPM). The document also includes information on senescence, apoptosis, and differentiation assays, as well as markers for early apoptosis and pro-differentiation.<br /><br />The document also provides clinical characteristics and spatial transcriptomics analysis of NUT Carcinoma. It includes information about the diagnosis, tumor size, stage, and immunohistochemistry (IHC) results. The IHC results indicate positive staining for P40, P63, TTF1, NUT, and cMYC, but negative staining for ALK. Tumor infiltrating lymphocytes are reported as moderate.<br /><br />The document also includes data on spatial gene expression and its clusters, as well as the top five markers for each cluster. It further provides information on NUTM1 targeting sgRNAs design, editing efficiency, mRNA and protein expression levels, and proliferation assay in a cell line called 10-15. The document also includes data on CRISPR/Cas9-mediated NUT suppression.<br /><br />Overall, this document presents a comprehensive analysis of NUT Carcinoma, providing information on gene expression, cellular assays, clinical characteristics, and targeted gene suppression techniques.

Asset Subtitle

Juyoung Choi

Meta Tag

Speaker Juyoung Choi

Topic Tumor Biology: Translational Biology - Omics and New Technologies

Keywords

NUT Carcinoma

primary lung cancer

RNA expression levels

genes

wild type

knockout

NUTM1 gene

transcripts per million

senescence

apoptosis