2023 World Conference on Lung Cancer (Posters)-P1.22. Identification of MIF as a Multiple Primary Lung Cancer Related Gene Based on RNA Sequencing Technology and Study of Its Function

P1.22. Identification of MIF as a Multiple Primary Lung Cancer Related Gene Based on RNA Sequencing Technology and Study of Its Function - PDF(Slides)

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Multiple primary lung cancer (MPLC) refers to the occurrence of two or more primary lung cancer lesions in the same patient. This study aimed to explore the transcriptome characteristics and related genes of MPLC through RNA sequencing technology. Tumor tissues and paired paracancerous tissues were collected from 18 MPLC patients and compared with data from the Cancer Genome Atlas (TCGA) database. The cell composition of MPLC tissues was described using single cell sequencing data. Differential gene expression analysis identified 1553 genes that were differentially expressed in MPLC compared to single lung adenocarcinoma. Single cell sequencing analysis revealed significant heterogeneity among different foci in MPLC and identified 18 subtypes of epithelial cells. The study further showed that the macrophage migration inhibitory factor (MIF) was a gene associated with MPLC, and its pathway was involved in the communication between various cell types. The overexpression of MIF was associated with a poor prognosis in lung adenocarcinoma. Additional experiments showed that the overexpression of MIF in AT2 cells led to morphological changes and improved proliferation, migration, and invasion abilities, suggesting a promoting effect on lung cancer. In conclusion, this study identified MIF as a gene associated with MPLC and provided insights into its role in lung cancer development. These findings offer a potential new approach for the diagnosis and treatment of MPLC.

Asset Subtitle

Haoshuai Yang

Meta Tag

Speaker Haoshuai Yang

Topic Pathology & Biomarkers: Genetic Biomarkers

Keywords

Multiple primary lung cancer

MPLC

transcriptome characteristics

related genes

RNA sequencing technology

differential gene expression analysis

heterogeneity

macrophage migration inhibitory factor

MIF

lung cancer development