2023 World Conference on Lung Cancer (Posters)-P1.24. Single-Cell Profiling Reveals the Trajectory of FOLR2+TAMs to Tregs in the Progression of Lung Adenocarcinoma

P1.24. Single-Cell Profiling Reveals the Trajectory of FOLR2+TAMs to Tregs in the Progression of Lung Adenocarcinoma - PDF(Slides)

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A study conducted at Shanghai Chest Hospital aimed to investigate the cellular mechanism underlying the formation of an immunosuppressive microenvironment in lung adenocarcinoma (LUAD). The researchers collected LUAD samples from patients at different pathological stages and performed single-cell RNA sequencing to analyze the transcriptomes of the cells. They identified seven major cell types, with myeloid cells and T cells being the most abundant. The frequency of some cell types varied along the progression of LUAD.<br /><br />The researchers found that FOLR2TAM, DCs, and NR4A3CD4T showed an enrichment in invasive adenocarcinoma (IA), suggesting their involvement in the progression of LUAD. The expression of FOLR2 was significantly higher in IA compared to earlier stages. The infiltration of NR4A3 and FOXP3 positive cells also increased during LUAD progression. The researchers hypothesized that FOLR2TAM elevation in IA might be partially due to the recruitment of DCs and other TAMs through chemokine recruitment.<br /><br />They further suggested that FOLR2TAM might collaborate with DCs to recruit NR4A3CD4T cells, which could potentially convert to regulatory CD4 T lymphocytes (Tregs) in the progression of LUAD. Pseudotime analysis supported the idea that NR4A3CD4T cells may differentiate into Tregs, as they expressed Treg differentiation-related genes. The study provided a single-cell transcriptome atlas of LUAD, highlighting the high cellular heterogeneity and transcriptomic features at different pathological stages.<br /><br />In conclusion, the study revealed a potential trajectory involving FOLR2TAM, DCs, NR4A3CD4T cells, and Tregs in shaping the immunosuppressive microenvironment in invasive LUAD. This understanding may have implications for developing targeted therapies to disrupt this trajectory and enhance immune responses against LUAD.

Asset Subtitle

Yuchen Han

Meta Tag

Speaker Yuchen Han

Topic Pathology & Biomarkers: Prognostic Biomarkers

Keywords

LUAD

single-cell RNA sequencing

FOLR2TAM

DCs

NR4A3CD4T

invasive adenocarcinoma

Tregs

chemokine recruitment

transcriptomic features

immune responses