2023 World Conference on Lung Cancer (Posters)-P2.15. Distinct Tumor Microenvironment in SCLC as compared with NSCLC by Single Cell Analysis

P2.15. Distinct Tumor Microenvironment in SCLC as compared with NSCLC by Single Cell Analysis - PDF(Abstract)

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In this study, the authors aimed to gain a deeper understanding of the molecular features and tumor microenvironment (TME) of small cell lung cancer (SCLC) compared to non-small cell lung cancer (NSCLC). They performed single-cell RNA sequencing on clinical specimens from SCLC, lung adenocarcinoma (LUAD), lung squamous cell carcinoma (LUSC), and normal lung tissues.<br /><br />The results showed that the proportions of cancer cells in the TME of SCLC were significantly higher compared to LUAD and normal lungs. SCLC had a higher percentage of CD4 naïve T cells, CD4 Th1-like T cells, and natural killer (NK) cells, while having a lower percentage of CD8 proliferating T cells and plasma cells compared to LUSC. When compared to LUAD, SCLC had a higher percentage of CD8 naïve T cells and a lower percentage of plasma cells.<br /><br />In addition, SCLC had higher levels of dendritic cells (DCs) and plasmacytoid DCs (pDCs), while showing lower levels of tumor-associated macrophages (TAMs) compared to LUAD and LUSC. The researchers also observed more frequent and dense interactions between SCLC cells and T cells and neutrophils, particularly CD8 effector T cells and CD8 naïve T cells. Pathway analyses revealed that myeloid cells in SCLC exhibited enrichment in neutrophil-associated pathways, which may contribute to immune escape in SCLC.<br /><br />In conclusion, the single-cell analysis conducted in this study revealed a distinct TME in SCLC, characterized by higher proportions of immune cells and specific cellular interactions. The findings suggest the potential significance of tumor microenvironment in the development and progression of SCLC.

Asset Subtitle

Xuanhe Wang

Meta Tag

Speaker Xuanhe Wang

Topic SCLC & Neuroendocrine Tumors: Preclinical

Keywords

tumor microenvironment

small cell lung cancer

non-small cell lung cancer

single-cell RNA sequencing

immune cells

CD4 T cells

CD8 T cells

plasma cells

dendritic cells

neutrophils