2023 World Conference on Lung Cancer (Posters)-P2.21. Characterization of the Genomic and Immune Landscape of Malignant Pleural Mesothelioma

P2.21. Characterization of the Genomic and Immune Landscape of Malignant Pleural Mesothelioma - PDF(Slides)

Pdf Summary

The goal of this study was to analyze the genomic and immune landscape of malignant pleural mesothelioma (MPM) in the context of immunotherapy. The researchers used Imaging Mass Cytometry (IMC) to analyze tissue samples from 22 MPM patients. They stained the samples with a panel of 27 metal-tagged antibodies to identify different cell phenotypes in the tumor microenvironment (TME). They found four major cell phenotypes in the TME.<br /><br />In addition, DNA from blood and tumor tissues of 16 patients was analyzed using whole exome sequencing (WES) to calculate the tumor mutational burden (TMB). They observed that both increased immune infiltrate and low TMB were associated with better overall survival. This suggests that the quality of mutations, rather than the quantity, drives an anti-tumor immune response and is associated with a more favorable prognosis.<br /><br />Furthermore, the researchers created heatmaps and UMAP plots to visualize the expression of proteins in different cellular clusters identified through IMC. They also analyzed the mutational profiles of MPM patients and found an inverse correlation between TMB and immune infiltrate. MPM patients with higher TMB had worse overall survival.<br /><br />Overall, this study provides insights into the genomic and immune landscape of MPM and its correlation with immunotherapy response. The findings suggest that immune infiltrate and TMB could serve as potential prognostic markers in MPM, helping to guide treatment decisions for patients. However, further research is required to validate these findings and explore potential therapeutic strategies targeting the immune microenvironment in MPM.

Asset Subtitle

Andrea Bille

Meta Tag

Speaker Andrea Bille

Topic Mesothelioma, Thymoma & Other Thoracic Tumors: Translational

Keywords

genomic analysis

immune landscape

malignant pleural mesothelioma

immunotherapy

tissue samples

cell phenotypes

tumor microenvironment

tumor mutational burden

immune infiltrate

overall survival