2024 Asia Conference on Lung Cancer (ACLC) - Poster & ePosters-PP01.45

PP01.45 - Qian Wang

Pdf Summary

The study investigates the effectiveness of carbon ion (C-ion) irradiation in inhibiting the cell viability of CD44A549 stem-like cells, a subset of cancer stem cells (CSCs) associated with non-small cell lung cancer (NSCLC). Conducted by researchers from the First School of Clinical Medicine, Lanzhou University, and the Institute of Modern Physics, Chinese Academy of Sciences, the study employs various methodologies to examine the impact of C-ion irradiation on cellular mechanisms.<br /><br />Using a fluorescence-activated cell sorter (FACS), the researchers sorted A549 and CD44A549 cell lines, subsequently subjecting them to ionizing radiation at doses of 0, 1, 2, and 4 Gy for C-ions and 0, 1, 2, 4, and 8 Gy for X-rays. The researchers assessed cell viability using CCK-8 and EdU assays. They also performed immunofluorescence to detect DNA double-strand breaks (DSBs) and Western blot analysis to probe protein expression levels. The study further assessed tumorigenicity through animal experiments.<br /><br />Key findings indicate that CD44A549 cells exhibit stem-like properties ('stemness') and demonstrate significantly higher radio-resistance compared to the A549 cell line. Importantly, the application of C-ion irradiation overcame this radio-resistance in CD44A549 cells, resulting in more substantial DNA double-strand breaks—a beneficial therapeutic outcome, considering the challenges presented by CSCs in cancer treatment.<br /><br />Statistical analysis using an unpaired two-tailed Student’s t-test and two-way ANOVA with Tukey’s multiple comparison test confirmed the significance of results, marking substantial findings with p-values: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Overall, the study positions C-ion irradiation as a promising approach to targeting radio-resistant cancer stem-like cells, potentially enhancing therapeutic efficacy against NSCLC.

Keywords

carbon ion irradiation

CD44A549 stem-like cells

cancer stem cells

non-small cell lung cancer

cell viability

DNA double-strand breaks

radio-resistance

tumorigenicity

therapeutic efficacy

Lanzhou University