2024 Asia Conference on Lung Cancer (ACLC) - Poster & ePosters-PP01.46

PP01.46 - Qian Wang

Pdf Summary

The study focuses on the role of autophagy in the radiation resistance observed in certain cell lines, specifically A549 and CD44A549. Using a range of methodologies, the research explores the cellular processes influencing radio-resistance. Key methods include using a fluorescence-activated cell sorter (FACS) for cell sorting, subjecting cells to varying doses of ionizing radiation (X-rays and C-ions), and assessing cell viability with CCK-8 and EdU assays. Metabolic differences were detected with Liquid Chromatography-Mass Spectrometry (LC–MS), while autophagy levels were measured using the CYTO-ID Autophagy Detection Kit 2.0 and Western blot analysis. The research also tested the effects of autophagy inhibitors and activators such as 3-MA and Rapamycin.<br /><br />Results from these methods indicate that CD44A549 cells exhibit greater radio-resistance compared to A549 cells. LC-MS analyses demonstrated considerable variation in autophagy levels, suggesting that autophagy contributes significantly to the radio-resistance of CD44A549 stem-like cells. The study concludes that the inhibition of autophagy can increase the radiation sensitivity of these cells, potentially offering a targeted therapeutic approach to enhance radiotherapy efficacy.<br /><br />Statistical analyses employed include unpaired two-tailed Student’s t-tests and two-way ANOVA with Tukey’s multiple comparison test, with significance levels marked from *P < 0.05 to ****P < 0.0001.<br /><br />In summary, the research suggests that modulating autophagy could be an effective strategy to counteract the radio-resistance of CD44A549 stem-like cells, providing insights into developing more effective cancer treatments.

Keywords

autophagy

radiation resistance

A549

CD44A549

FACS

ionizing radiation

LC-MS

autophagy inhibitors

radio-resistance

cancer treatments