2024 Asia Conference on Lung Cancer (ACLC) - Poster & ePosters-PP01.47

PP01.47 - Yuan Xu

Pdf Summary

The study aimed to identify crucial genes within the competing endogenous RNA (ceRNA) network of lung adenocarcinoma (LUAD) that can potentially influence patient prognosis at the single-cell level. The researchers constructed a ceRNA regulatory network by performing RNA sequencing on LUAD samples and integrating this data with public databases. This approach allowed them to pinpoint key pathways associated with patient outcomes and reveal the expression levels of significant genes under various conditions. They also explored the functional enrichment of co-expressed genes and their potential immune-related mechanisms.<br /><br />The study discovered that the ZC3H12D-hsa-miR-4443-ENST00000630242 axis is linked with LUAD. Notably, lower expression of ZC3H12D was significantly associated with reduced overall survival in patients, suggesting it may serve as a prognostic marker. Single-cell RNA sequencing showed that ZC3H12D was more highly expressed in CD8 and CD4 T cells, as well as B cells, in LUAD. Furthermore, functional enrichment analysis indicated that the co-expressed genes are primarily involved in lymphocyte activation and cytokine-cytokine receptor interactions. The study found that ZC3H12D is related to multiple immune cells and molecules, including immune checkpoints like CTLA4, CD96, and TIGIT.<br /><br />In conclusion, the research identified the ZC3H12D-hsa-miR-4443-ENST00000630242 ceRNA network in LUAD. The findings suggest that ZC3H12D affects patient prognosis by regulating a network of mRNA, miRNA, lncRNA, immune cells, and immune molecules, highlighting its potential as a target for LUAD prognosis.

Keywords

lung adenocarcinoma

ceRNA network

ZC3H12D

single-cell RNA sequencing

prognostic marker

immune-related mechanisms

lymphocyte activation

cytokine-cytokine receptor interactions

immune checkpoints

patient prognosis