2024 Asia Conference on Lung Cancer (ACLC) - Poster & ePosters-PP02.08

PP02.08 - Huiqing Lin

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The study investigates the immune microenvironment in multiple primary lung cancer (MPLC) using integrated single-cell RNA sequencing (scRNA-seq) and spatial transcriptomic sequencing. Conducted by researchers from Renmin Hospital, Wuhan University, the research aims to enhance understanding of MPLC, a condition whose diagnosis and distinction from metastatic lung cancer remains complex due to its intricate changes in tumor cell composition.<br /><br />Utilizing whole exome sequencing and spatial transcriptome techniques, the study analyzed 22 MPLC-identified samples from public single-cell databases alongside those newly sequenced. This integration revealed significant variations in gene expression among MPLC patient samples, highlighting macrophage and plasma cell marker genes like MYL9, CXCL2, and PDK4. These findings are crucial for revealing distinct immune patterns in the tumor microenvironment related to different cancer progression foci.<br /><br />Moreover, the study examined 84,419 cells, classifying them into 14 cell clusters visualized via tSNE and UMAP analyses. This highlighted structural variations in the transcriptomic landscape, providing novel insights into immune cell infiltration and distinct molecular markers within MPLC. <br /><br />The significant outcomes of the study include the identification of immune cell markers and the elucidation of different regulatory pathways through KEGG and gene ontology enrichment analyses. The results suggest varied immune responses credited to different cancer progression stages, aiding in distinguishing MPLC foci like adenocarcinoma in situ (AIS), invasive adenocarcinoma (IA), and microinvasive adenocarcinoma (MIA).<br /><br />Conclusively, this research advances understanding of MPLC's immune microenvironment, proposing key target genes that could guide more accurate future diagnoses and treatments. These findings contribute substantially to the comprehension of MPLC's underlying immunological mechanisms and their impact on tumor progression.

Keywords

immune microenvironment

multiple primary lung cancer

MPLC

single-cell RNA sequencing

spatial transcriptomic sequencing

gene expression

macrophage markers

immune cell infiltration

tumor microenvironment

cancer progression