2024 Asia Conference on Lung Cancer (ACLC) - Poster & ePosters-PP02.60

PP02.60 - Shaodi Wen

Pdf Summary

The study explores the role of ALCAM (Activated Leukocyte Cell Adhesion Molecule) and its interaction with T cells in early recurrent metastasis of stage III non-small cell lung cancer (NSCLC). Researchers investigated how ALCAM expression affects tumor and immune interactions, utilizing advanced single-cell sequencing and other molecular techniques.<br /><br />Key findings include: <br />1. **ALCAM's Role in Metastasis:** High ALCAM levels in primary tumors are linked to poor overall survival, suggesting a dual function in tumor progression. The loss of ALCAM seems to promote a shift towards a more aggressive cancer phenotype by influencing cell cycle pathways and enhancing cellular migration and invasion capacities. <br /><br />2. **ALCAM and the Immune Microenvironment:** The study revealed changes in the communication between epithelial cells and T cells due to the altered expression of ALCAM, leading to decreased interactions with CD6, a receptor on T cells. These alterations may contribute to the immune system's compromised ability to control tumor growth.<br /><br />3. **Experimental Validation:** Experiments using ALCAM-silenced lung cancer cell lines showed increased metastatic potential, reflecting the disruption in the tumor microenvironment due to ALCAM downregulation. Additionally, silencing MYB, a regulator of ALCAM, supported these findings by further diminishing ALCAM expression and enhancing tumor progression.<br /><br />4. **Pseudotime Analysis and Gene Regulation:** The study highlighted the critical pathways and initial cellular changes that lead to metastatic recurrence in NSCLC by identifying specific cell types and transcription factors involved.<br /><br />The research concludes that ALCAM plays a significant role in NSCLC's metastatic recurrence and survival outcomes. While the current findings need validation through larger-scale studies, they provide a foundation for developing targeted therapies and predictive tools based on the ALCAM-CD6 axis in managing NSCLC.

Keywords

ALCAM

T cells

metastasis

NSCLC

immune microenvironment

single-cell sequencing

tumor progression

CD6

gene regulation

targeted therapies