2024 World Conference on Lung Cancer (WCLC) - Posters-P3.02F.05 A Genetic Engineering Approach to Interrogate Myosin 10 As a Driver of Non-Small Cell Lung Cancer Metastasis

P3.02F.05 A Genetic Engineering Approach to Interrogate Myosin 10 As a Driver of Non-Small Cell Lung Cancer Metastasis

Pdf Summary

This study explores the role of Myosin 10 (MYO10) in driving metastasis in non-small cell lung cancer (NSCLC) using genetic engineering. The researchers highlight the challenge of late-stage lung cancer diagnosis, which significantly decreases survival rates. NSCLC cells have subpopulations with varied metastatic potentials, notably the "Leaders," highly metastatic cells with elevated MYO10 expression. The study's objective is to elucidate MYO10's involvement in the metastatic business, particularly focusing on Leader cells.<br /><br />Using the CRISPR-Cas9 system, MYO10 knockout (KO) was achieved in H1299 NSCLC Leader cells, creating MYO10-/- clones. These clones exhibited slower proliferation, reduced size, and diminished force generation compared to their MYO10-expressing counterparts, yet retained similar integrin expression profiles. This suggests that while MYO10 impacts force generation and cell growth, it doesn't significantly alter integrin subunit levels.<br /><br />The research also aims to investigate differences in apoptosis, invasion, and other cellular processes by comparing MYO10-/- and MYO10/+ clones, supplemented by bulk RNA sequencing to uncover transcriptional changes. This investigation provides foundational insights into how MYO10 contributes to Leader cell behavior and metastasis, offering a model to further explore MYO10-dependent mechanisms driving cancer invasion.<br /><br />This work is supported by national funding and involves collaborative efforts from various departments at Emory University. It sets the stage for future research aimed at better understanding metastatic mechanisms, potentially leading to improved strategies for managing and treating metastatic lung cancer.

Asset Subtitle

Raaven Goffe

Meta Tag

Speaker Raaven Goffe

Topic Tumor Biology – Preclinical Biology

Keywords

Myosin 10

NSCLC

metastasis

genetic engineering

CRISPR-Cas9

Leader cells

H1299

RNA sequencing

integrin expression

Emory University