2024 World Conference on Lung Cancer (WCLC) - Posters-P3.03H.01 Tumour Educated Platelets Display A Distinct RNA Content Profile Across Metastatic Patterns in NSCLC.

P3.03H.01 Tumour Educated Platelets Display A Distinct RNA Content Profile Across Metastatic Patterns in NSCLC.

Pdf Summary

This study investigates the RNA content of tumor-educated platelets (TEPs) in Stage IV non-small cell lung cancer (NSCLC) patients, highlighting their distinct gene signature profiles across various metastatic sites. TEPs, which are platelets that interact with tumor cells, can undergo changes in RNA and protein content. The study used a dataset (GSE183634) from the Gene Expression Omnibus, including 399 Stage IV NSCLC patients, 33 early-stage NSCLC patients, and 390 asymptomatic controls. Differential gene expression analysis was performed with edgeR, and gene set enrichment analysis utilized clusterProfiler, correlated via the TCGA LUAD dataset.<br /><br />Key findings show that TEPs from Stage IV NSCLC patients exhibit upregulation of immune and metabolic pathways compared to controls, which are evident in altered biological processes in platelet RNA content. Different metastatic sites like brain, bone, liver, and adrenal glands revealed unique and overlapping altered genes, with significant differences in immune and tumor progression pathways.<br /><br />The study emphasizes that RNA content in TEPs varies notably with the metastatic pattern in advanced NSCLC, as compared to early-stage disease. Unique and common RNA changes were observed, demonstrating varied biological processes involved in metastasis. These RNA signatures indicate alterations in processes related to metabolism, transcription, translation, and post-transcriptional modifications.<br /><br />This research underscores the potential of investigating TEP RNA content to elucidate mechanisms of metastatic progression and identify anti-metastatic targets. The distinct TEP profiles could help predict early-stage patients who are at risk of recurrence, providing a foundation for targeted interventions and therapeutic strategies.

Asset Subtitle

Anne-Marie Baird

Meta Tag

Speaker Anne-Marie Baird

Topic Tumor Biology – Translational Biology

Keywords

tumor-educated platelets

Stage IV NSCLC

RNA content

gene signature

metastatic sites

differential gene expression

immune pathways

metabolic pathways

metastatic progression

therapeutic strategies