2024 World Conference on Lung Cancer (WCLC) - Posters-P3.03H.05 AKAP12 Deficiency Leads to Blood-Brain Barrier Disruption and Serves as a Crucial Diagnostic Marker for Brain Metastasis in Lung Adenocarcinoma

P3.03H.05 AKAP12 Deficiency Leads to Blood-Brain Barrier Disruption and Serves as a Crucial Diagnostic Marker for Brain Metastasis in Lung Adenocarcinoma

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The study "AKAP12 Deficiency Leads to Blood-Brain Barrier Disruption and Serves as a Crucial Diagnostic Marker for Brain Metastasis in Lung Adenocarcinoma" investigates the link between AKAP12 deficiency and brain metastasis in lung adenocarcinoma. AKAP12 plays a vital role in maintaining the blood-brain barrier (BBB), which gets disrupted during brain metastasis of lung adenocarcinoma. The research emphasizes that the lack of AKAP12 contributes to the degradation of the BBB, thereby facilitating the metastatic process.<br /><br />The study's methodology included single-cell RNA sequencing to identify differentially expressed genes in primary versus metastatic lung cancer cells. Researchers utilized tissue microarrays and multiplex immunohistochemistry to analyze lung adenocarcinoma brain metastases. They also developed a zebrafish model using Crispant technology to understand the role of the AKAP12b gene in BBB integrity. Quantitative proteomics was employed to measure protein levels in extracellular vesicles (EVs) in plasma, confirming the differential presence of AKAP12.<br /><br />Notably, analyses revealed reduced AKAP12 expression in extracellular vesicles from lung adenocarcinoma patients compared to healthy individuals. This makes AKAP12 a promising biomarker for diagnosing brain metastasis by indicating BBB deterioration. The results highlighted poor prognoses associated with brain metastasis in lung adenocarcinoma, affecting about 50% of patients.<br /><br />In conclusion, the study identifies AKAP12 within EVs as a critical diagnostic marker for brain metastasis in lung adenocarcinoma, underlining its potential utility in predicting metastatic events and guiding therapeutic strategies. The findings underscore the necessity to develop biomarkers for accurately assessing and managing brain metastasis in affected patients.

Asset Subtitle

Xie Tian

Meta Tag

Speaker Xie Tian

Topic Tumor Biology – Translational Biology

Keywords

AKAP12 deficiency

blood-brain barrier

brain metastasis

lung adenocarcinoma

single-cell RNA sequencing

tissue microarrays

zebrafish model

extracellular vesicles

biomarker

diagnostic marker