2024 World Conference on Lung Cancer (WCLC) - Posters-P3.06D.14 Liquid Biopsy-Based Single T-Cell Dynamic Polyfunctionality Profiling as a NSCLC Immunotherapy Biomarker

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The document presents a study on a novel approach to predict lung cancer patient responses to immune checkpoint inhibitors (ICIs) by evaluating a live single-cell functional liquid biopsy platform, IsoLight. Traditional predictive biomarkers, like PD-L1 tumor proportional score (TPS), have limited clinical applications and require invasive biopsies. These are especially challenging for longitudinal monitoring and become less useful when ICIs are combined with chemotherapies. The study introduces peripheral single T-cell dynamic polyfunctionality profiling as a less invasive yet potentially more effective method for tracking the molecular responses to ICIs in non-small cell lung cancer (NSCLC).<br /><br />The research involved collecting peripheral blood mononuclear cell samples from 10 healthy donors and 10 NSCLC patients undergoing ICI-based therapies. The IsoLight platform was employed to perform cytokine profiling of these cells, specifically looking at CD4 and CD8 T-cells, to evaluate treatment responses pre- and post-therapy.<br /><br />Key findings showed that changes in CD8 T-cell polyfunctionality significantly correlated with ICI treatment responses, suggesting that CD8 ΔPSI and PD-L1 TPS combined scores could serve as strong predictors of early ICI treatment efficacy. Additionally, while the changes in the polyfunctionality strength index (ΔPSI) for CD4 T-cells weren't significant predictors of immediate treatment response, they showed a strong trend in correlating with long-term clinical survival outcomes, such as progression-free survival (PFS) and overall survival (OS).<br /><br />Overall, the results support the potential of IsoLight's dynamic monitoring of T-cell polyfunctionality as a predictive biomarker for both ICI treatment response and clinical outcomes in NSCLC. The findings propose the advantage of this method over traditional tissue-based scores, with ongoing and future research suggested to validate these results in larger cohorts.

Asset Subtitle

Zuan-Fu Lim

Meta Tag

Speaker Zuan-Fu Lim

Topic Pathology & Biomarkers

Keywords

lung cancer

immune checkpoint inhibitors

IsoLight platform

single-cell functional liquid biopsy

non-small cell lung cancer

T-cell polyfunctionality

CD8 T-cells

predictive biomarkers

treatment response

clinical outcomes