2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.02B.06 Analysis on the Correlation Between RUNX3 Mislocalization and Epigenetic Parameters in Non-Small Cell Lung Cancer

EP.02B.06 Analysis on the Correlation Between RUNX3 Mislocalization and Epigenetic Parameters in Non-Small Cell Lung Cancer

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The study led by Weibin Liu and his team at Fujian Medical University investigates the epigenetic factors influencing the mislocalization of RUNX3 in non-small cell lung cancer (NSCLC). RUNX3, initially identified in gastric disorders, functions mainly as a tumor suppressor but can exhibit oncogenic activity depending on its interaction with signaling molecules. Previous research highlighted RUNX3's expression in NSCLC as negative, nuclear, cytoplasmic, or whole-cell, with non-nuclear expression being linked to postoperative metastasis.<br /><br />The study utilized tissue microarrays composed of samples from 205 NSCLC patients and 5 normal lung tissues to evaluate epigenetic indicators such as H3K27me3, H2AK119U1, EZH2, DNMT1/3b, TGF-β, and c-myc. Analytical methods included Western blotting to assess protein levels and pyrosequencing to measure methylation at the RUNX3 promoter.<br /><br />The findings revealed significant variations in the expression of epigenetic markers across RUNX3 expression patterns (negative, nuclear, cytoplasmic, whole-cell). Specifically, non-nuclear localization of RUNX3 was associated with diminished expression of H3K27me3, EZH2, H2AK119U1, c-myc, and DNMT3b. Additionally, higher methylation levels at the RUNX3 promoter's CpG sites were linked to lower RUNX3 expression and an increased risk of local relapse post-surgery.<br /><br />In conclusion, the study suggests that epigenetic mechanisms contribute to RUNX3 mislocalization. Specifically, RUNX3 promoter hypermethylation is significantly correlated with relapse in NSCLC, underscoring the importance of regulating RUNX3 expression for accurate prognosis and treatment strategies.

Asset Subtitle

weibin liu

Meta Tag

Speaker weibin liu

Topic Tumor Biology – Preclinical Biology

Keywords

RUNX3

NSCLC

epigenetic factors

mislocalization

H3K27me3

methylation

EZH2

DNMT3b

c-myc

prognosis