2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.02F.02 Individual and Combined Effects of Annonacin and 2-Deoxy-D-Glucose on Non-Small Cell Lung Cancer Cells

EP.02F.02 Individual and Combined Effects of Annonacin and 2-Deoxy-D-Glucose on Non-Small Cell Lung Cancer Cells

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The study aims to explore the effects of two compounds, Annonacin and 2-Deoxy-D-Glucose (2DG), on non-small cell lung cancer (NSCLC) cells, specifically the A549 cell line. NSCLC is the most common type of lung cancer and a leading cause of cancer-related mortality globally and in Kentucky. The researchers focus on disrupting cancer cell metabolism, particularly targeting glycolysis and mitochondrial function, which are critical for cancer cell energy production.<br /><br />The study hypothesizes that Annonacin (a mitochondrial complex I inhibitor) and 2DG (a glycolytic inhibitor) will individually and collectively lead to significant inhibition of A549 cell growth. The anticipated mechanisms include increased oxidative stress and DNA damage, resulting in reduced cell viability. The research utilizes in vitro methods, employing the A549 NSCLC cell line and the NL20 bronchial epithelial cell line as a control.<br /><br />Two assays are used to assess the effects of these treatments: the MTT assay for cell viability and cytotoxicity, and the Colony Forming Assay (CFA) for long-term proliferation capacity. Results indicate that both Annonacin and 2DG have synergistic cytotoxic effects on A549 cells, with impacts being dose and time-dependent. The disruption of essential metabolic pathways causes decreased cell viability, supporting the idea of metabolic reprogramming as a therapeutic target in NSCLC.<br /><br />Future work involves understanding the molecular mechanisms underlying the observed effects, focusing on roles like oxidative stress and genotoxicity through additional assays. The study is funded by the USDA's Evans-Allen Project, reflecting the ongoing exploration of NSCLC treatment options by leveraging metabolic disruptions.

Asset Subtitle

Bhoj Raj Bhattarai

Meta Tag

Speaker Bhoj Raj Bhattarai

Topic Tumor Biology – Preclinical Biology

Keywords

Annonacin

2-Deoxy-D-Glucose

NSCLC

A549 cell line

cancer metabolism

glycolysis inhibition

mitochondrial function

cell viability

oxidative stress

metabolic reprogramming