2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.02F.03 Elucidating the Role of XRCC6BP1 In Mitochondrial Bioenergetics and Metabolism in Non-Small Cell Lung Cancer

EP.02F.03 Elucidating the Role of XRCC6BP1 In Mitochondrial Bioenergetics and Metabolism in Non-Small Cell Lung Cancer

Pdf Summary

The study by Martin P. Barr explores the role of XRCC6BP1, a protein involved in DNA double-stranded break repair, focusing on its influence on mitochondrial function and metabolism in non-small cell lung cancer (NSCLC). The research investigates whether XRCC6BP1 impacts mitochondrial bioenergetics and treatment resistance by evaluating its effect on gene expression, cell metabolism, and apoptosis in NSCLC cells.<br /><br />Key findings show that knockdown of XRCC6BP1 leads to differential expression of mitochondrial genes, with a notable increase in the expression of UCP2, particularly in A549 cell lines, hinting at a potential role in managing oxidative stress and treatment resistance. Interestingly, the study found that XRCC6BP1 knockdown did not significantly affect apoptosis rates or alter the metabolic parameters of NSCLC cells, which could imply that while XRCC6BP1 influences gene expression, it may not directly alter cell survival or energy metabolism.<br /><br />The research highlights significant metabolic differences between adenocarcinoma (A549) and squamous cell carcinoma (SKMES-1) NSCLC cell lines, where A549 cells generally displayed higher levels of certain metabolic activities like Basal Respiration and ATP-Production but lower Spare Respiratory Capacity. These findings may help understand the variable treatment responses between different NSCLC subtypes.<br /><br />Overall, this study suggests that XRCC6BP1 plays a role in the metabolic reprogramming of NSCLC but does not affect apoptosis or general metabolism. UCP2's upregulation associated with XRCC6BP1 knockdown and cisplatin resistance points to future studies focusing on mitochondrial roles in cancer resistance mechanisms and may highlight new therapeutic targets. This work lays the groundwork for further investigation into mitochondrial behavior in drug-resistant NSCLC cells.

Asset Subtitle

Martin Barr

Meta Tag

Speaker Martin Barr

Topic Tumor Biology – Preclinical Biology

Keywords

XRCC6BP1

DNA repair

mitochondrial function

NSCLC

gene expression

cell metabolism

UCP2

oxidative stress

adenocarcinoma

cisplatin resistance