2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.04C.09 Liquid Biopsy Assay to Detect Low Levels of ctDNA in Early-Stage Lung Cancer Samples

Pdf Summary

The study by Genece Health on their Lung Cancer Assay evaluates the assay's sensitivity in detecting circulating tumor DNA (ctDNA) for early-stage lung cancer, even with minimal cell-free DNA (cfDNA) quantities from blood samples. The assay demonstrates an 89.5% detection sensitivity for early-stage lung cancer samples, particularly on undiluted samples. Testing was conducted on cfDNA inputs ranging from 0.25ng to 2ng, extracted from a single tube of blood. Samples with sufficient presence of cfDNA successfully demonstrated the assay's efficacy at very low input concentrations.<br /><br />Key findings include:<br />- Detection of 17 out of 19 samples when cfDNA was not diluted, with an overall assay sensitivity of 89.5%.<br />- At a 2ng input level, all tested samples with adequate cfDNA were detected.<br />- Sensitivity dropped with reduced cfDNA inputs: 53% detection at 1ng and 67% at 0.5ng. Attempts at 0.25ng input failed due to issues in library preparation.<br /><br />The research acknowledges the challenge of detecting ctDNA due to its low concentration in early-stage cancers, a critical task given that earlier cancer detection correlates with significantly higher survival rates. Advanced technologies focus on maximizing the usage of small ctDNA fractions to identify cancer presence.<br /><br />Additionally, the study tested the reproducibility of results across input ranges and found consistent detection in triplicate runs of several samples, even at low input. Through advanced analysis techniques, including fragment length and motif analysis, the assay can maintain stability and accuracy in cancer prediction. This capacity positions the Genece Health Lung Cancer Assay as a promising tool for early detection of lung cancer, leveraging minimal patient input and comprehensive genomic assessment.

Asset Subtitle

Kristin Fathe

Meta Tag

Speaker Kristin Fathe

Topic Screening and Early Detection

Keywords

Lung Cancer Assay

Genece Health

circulating tumor DNA

ctDNA detection

cell-free DNA

early-stage cancer

sensitivity

genomic assessment

fragment length analysis

cancer prediction