2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.06F.04 The Characteristics of Plasma Sample NGS Analysis in the Detection of Lung Cancer Driver Genes

EP.06F.04 The Characteristics of Plasma Sample NGS Analysis in the Detection of Lung Cancer Driver Genes

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The study investigates the effectiveness of plasma sample Next-Generation Sequencing (NGS) analysis in detecting lung cancer driver genes, focusing on its potential to replace traditional tissue specimens for non-small cell lung cancer (NSCLC) cases. The advantages of using plasma samples include minimal invasiveness, convenience, and quicker result turnaround times.<br /><br />The study was conducted across six Japanese institutions, enrolling 303 suspected lung cancer cases with 261 being diagnosed as NSCLC. Using the Genexus system and Oncomine Precision Assay, plasma samples yielded NGS results for 261 out of 261 cases, whereas tissue samples were successful in 214 out of 261 cases. Additionally, the plasma NGS had a faster turnaround time (8 days on average) compared to tissue samples (20 days on average).<br /><br />Analysis showed that driver genes were detected in both tissue and plasma samples in about 51.7% of cases. However, only a few genes were exclusively identified in plasma samples. The detection rate for specific genes like EGFR, ALK, and others showed a variable agreement between tissue and plasma samples, with a better correlation for mutations than gene fusions (55.9% vs 34.8%).<br /><br />In conclusion, while the plasma NGS offers faster results with the benefit of being less invasive, it detects driver genes in fewer cases compared to tissue analysis. Nonetheless, it serves as a promising tool for rapid and less invasive lung cancer diagnostics, meriting further optimization and exploration.

Asset Subtitle

Ken Uchibori

Meta Tag

Speaker Ken Uchibori

Topic Pathology and Biomarkers

Keywords

plasma sample

Next-Generation Sequencing

lung cancer

driver genes

non-small cell lung cancer

NSCLC

Genexus system

Oncomine Precision Assay

EGFR

ALK