2024 World Conference on Lung Cancer (WCLC) - ePosters-EP.06G.15 Rapid Immunohistochemistry of Microsatellite Instability Using Non-Contact Alternating Current Electric Field Mixing

EP.06G.15 Rapid Immunohistochemistry of Microsatellite Instability Using Non-Contact Alternating Current Electric Field Mixing

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The study by Kazuhiro Imai and colleagues presents a novel method of rapid immunohistochemistry (IHC) to assess microsatellite instability (MSI) in tumors, aimed at providing accurate intraoperative analysis using non-contact alternating current (AC) electric field mixing. This method focuses on the detection of deficiencies in mismatch repair (MMR) proteins, which are crucial for distinguishing MSI-high cancers—known to be more responsive to immuno-oncology therapies—from others.<br /><br />The rapid IHC technique, facilitated by AC mixing, is designed to shorten the processing time to within 40 minutes, compared to the over 3.5 hours required by conventional methods. AC mixing induces electro-osmotic vortices, enhancing the interaction between antigens and antibodies without the need for mechanical stirring. This method maintains or surpasses the staining quality of conventional IHC, as demonstrated in studies with both formalin-fixed paraffin-embedded (FFPE) and frozen tissue sections.<br /><br />The research involved validating this rapid IHC method on a cohort of patients, observing complete consistency in MMR protein detection between this new technique and traditional methods. The study highlights that rapid IHC could effectively guide immunotherapy strategies for MSI-high/dMMR tumors by allowing timely and precise molecular characterization during surgery.<br /><br />The conclusion emphasizes the practical benefits of this rapid IHC technique, such as its ease of use, high accuracy, and minimal tissue disturbance, making it a valuable tool for surgical pathology and subsequent molecular assessments. This could significantly impact clinical decisions, particularly in selecting appropriate immunotherapy regimens for patients with these genetic profiles.

Asset Subtitle

Kazuhiro Imai

Meta Tag

Speaker Kazuhiro Imai

Topic Pathology and Biomarkers

Keywords

rapid immunohistochemistry

microsatellite instability

non-contact AC electric field

mismatch repair proteins

MSI-high cancers

immuno-oncology therapies

electro-osmotic vortices

formalin-fixed paraffin-embedded

molecular characterization

surgical pathology