WCLC 2025 - Posters & ePosters-EP.06.30 Single-Cell RNA Sequencing Reveals a Subset of FSIP1 Cancer Cells and Verified Its Value of Prognosis in Lung Adenocarcinoma

EP.06.30 Single-Cell RNA Sequencing Reveals a Subset of FSIP1 Cancer Cells and Verified Its Value of Prognosis in Lung Adenocarcinoma

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This study investigates the role of Fibrous Sheath-Interacting Protein 1 (FSIP1) in lung adenocarcinoma (LUAD), the most common subtype of non-small cell lung cancer (NSCLC). FSIP1, originally known as a component of sperm flagellum structure, has been implicated in promoting tumor growth, invasion, and drug resistance in various cancers, but its function and prognostic value in LUAD were previously unclear.<br /><br />Using single-cell RNA sequencing data from early-stage resectable LUAD samples, researchers identified a distinct cancer cell subset with high FSIP1 expression. Analysis of 180 LUAD tissue samples showed that elevated FSIP1 protein levels are significantly associated with poorer patient survival, confirmed via Kaplan-Meier analysis and Cox regression models. A prognostic nomogram incorporating FSIP1 expression effectively predicted patient outcomes, supported by Receiver Operating Characteristic (ROC) curves.<br /><br />Functional assays using FSIP1-silenced LUAD cell lines (A549 and H1975) demonstrated markedly reduced migration ability, as evidenced by wound-healing and Transwell migration assays. Western blot analyses revealed that FSIP1 knockdown suppresses the PI3K/AKT signaling pathway, a critical pathway involved in cancer progression.<br /><br />In summary, the study identifies FSIP1 as a novel prognostic biomarker for LUAD patients, where high expression correlates with worse survival. Furthermore, FSIP1 promotes LUAD cell migration and activates oncogenic PI3K/AKT signaling, suggesting it as a potential therapeutic target. These findings were supported by data from cell-based experiments and patient tissue analyses. The research was funded by health and science foundations in Hangzhou and Zhejiang, China, with no conflicts of interest declared.<br /><br />Presented at the IASLC World Conference on Lung Cancer 2025, this work highlights FSIP1’s significance in LUAD prognosis and its molecular role in tumor cell behavior.

Asset Subtitle

Shirong Zhang

Meta Tag

Speaker Shirong Zhang

Topic Pathology and Biomarkers

Keywords

FSIP1

lung adenocarcinoma

LUAD

non-small cell lung cancer

NSCLC

prognostic biomarker

PI3K/AKT signaling pathway

cancer cell migration

single-cell RNA sequencing

tumor progression