2023 World Conference on Lung Cancer (Posters)-EP02.01. Androgen Receptor Suppresses Lung Cancer Invasion and Cisplatin Resistance via Decreasing TPD52 Expression

EP02.01. Androgen Receptor Suppresses Lung Cancer Invasion and Cisplatin Resistance via Decreasing TPD52 Expression - PDF(Abstract)

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This abstract discusses a study on the role of androgen receptor (AR) in lung cancer invasion and cisplatin resistance. The researchers aimed to explore the mechanisms by which AR impacts the progression of non-small-cell lung cancer (NSCLC), particularly its connection to TPD52-mediated cell invasion and resistance to cisplatin.<br /><br />The study used various methods such as plasmid construction, MTT assay, Transwell invasion assay, Western blot assay, qRT-PCR assay, chromatin immunoprecipitation assay (ChIP), luciferase reporter assay, in vivo studies, and statistical analysis.<br /><br />The results showed that AR can suppress NSCLC cell invasion and cisplatin resistance by decreasing the expression of a circular RNA called circ-SLCO1B7. AR directly binds to the 5' promoter region of its host gene SLCO1B7 to suppress circ-SLCO1B7. This circ-SLCO1B7 acts as a sponge to inhibit the function of miR-139-5p, a micro RNA. The inhibition of miR-139-5p leads to increased translation of Tumor protein D52 (TPD52) messenger RNA by reducing miRNA binding to its 3' untranslated region (3'UTR).<br /><br />The researchers confirmed these findings using an in vivo mouse model with orthotopic xenografts of NSCLC cells, as well as analysis of human NSCLC databases and clinical specimens.<br /><br />Overall, the study suggests that AR plays a role in suppressing NSCLC cell invasion and drug resistance by modulating the AR/ circ-SLCO1B7/ miR-139-5p/ TPD52 signaling pathway. Targeting this signaling pathway may lead to the development of new treatment strategies to better suppress NSCLC progression.

Asset Subtitle

Shiqing Liu

Meta Tag

Speaker Shiqing Liu

Topic Tumor Biology: Preclinical Biology - Molecular Therapeutic Targets

Keywords

androgen receptor

lung cancer invasion

cisplatin resistance

non-small-cell lung cancer

TPD52-mediated cell invasion

circ-SLCO1B7

miR-139-5p

Tumor protein D52

AR/ circ-SLCO1B7/ miR-139-5p/ TPD52 signaling pathway

NSCLC progression