2023 World Conference on Lung Cancer (Posters)-EP02.01. CSNK1D and CSNK1E Inhibition Regulates Autophagy by Promoting Autophagic Flux in Lung Cancer

EP02.01. CSNK1D and CSNK1E Inhibition Regulates Autophagy by Promoting Autophagic Flux in Lung Cancer - PDF(Slides)

Pdf Summary

A study conducted by researchers at Shenzhen University Health Science Center in China has revealed that the inhibition of Casein Kinase I (CK1) promotes autophagy in lung cancer cells. Autophagy is a cellular process that helps in maintaining cellular homeostasis by recycling and reorganizing cellular components. It plays a crucial role in the development and treatment of cancer.<br /><br />The study found that CK1 isoforms play an important role in modulating autophagy in lung cancer. Specifically, the inhibition of CK1 resulted in an increase in autophagic flux in lung cancer cells. The researchers observed this increase through the use of a mCherry-eGFP-LC3 reporter system, which detects autophagic flux by quenching the green fluorescence in acidic organelles. They also observed an autophagic phenotype and morphology in lung cancer cells when CK1 expression was inhibited.<br /><br />Furthermore, the researchers found that inhibiting CK1 led to a decrease in the levels of SQSTM1/p62 protein and an increase in the ratio of LC3II/I, indicating the induction of autophagy. The mRNA levels of autophagy indicators SQSTM1/p62 and LC3 remained unchanged under CK1 inhibition, suggesting that the decrease in SQSTM1/p62 protein levels and the increase in lipidated LC3 are regulated by the autophagy and autolysosomal pathway.<br /><br />The study provides valuable insights into the involvement of CK1 in cancer cell autophagy. The findings suggest that inhibiting CK1 can enhance autophagy in lung cancer cells. This discovery could potentially lead to the development of novel therapeutic strategies for lung cancer treatment. The study was supported by the National Natural Science Foundation of China, and the researchers expressed gratitude to the Instrument Analysis Center of Shenzhen University for their assistance with confocal analysis.

Asset Subtitle

Vivian Weiwen Xue

Meta Tag

Speaker Vivian Weiwen Xue

Topic Tumor Biology: Preclinical Biology - Molecular Therapeutic Targets

Keywords

Casein Kinase I

autophagy

lung cancer cells

cellular homeostasis

cancer development

cancer treatment

mCherry-eGFP-LC3 reporter system

autophagic flux

SQSTM1/p62 protein

LC3II/I ratio