2023 World Conference on Lung Cancer (Posters)-EP02.01. Suppression of Telomerase RNA Component Induces Autophagy and Ferroptosis in Lung Cancer via Regulation of AMP-actived Protein Kinase

EP02.01. Suppression of Telomerase RNA Component Induces Autophagy and Ferroptosis in Lung Cancer via Regulation of AMP-actived Protein Kinase - PDF(Slides)

Back to course

Pdf Summary

Long non-coding RNAs (lncRNAs) play a role in the development of tumors, including lung adenocarcinoma (LUAD). One specific lncRNA called telomerase RNA component (TERC) is important for the addition of telomere repeats, and dysfunction of TERC has been linked to various human diseases. However, the impact of TERC dysregulation on LUAD progression is not well understood.<br /><br />This study aimed to investigate the effects of TERC expression and activity on LUAD. The researchers used RNA sequencing to compare the levels of TERC in cancerous and normal lung tissues. They also performed functional assays in LUAD cell lines using siRNA to knockdown TERC expression.<br /><br />The results showed that TERC was upregulated in lung cancer tissues and LUAD cell lines. Knockdown of TERC inhibited the growth, migration, and invasion of LUAD cells. Additionally, TERC knockdown induced autophagy, as evidenced by increased expression of autophagy-related proteins and the formation of autophagosomes. It also activated ferroptosis, a form of cell death involving the accumulation of reactive oxygen species (ROS). Knockdown of TERC reduced the expression of ferroptosis inhibitors and resulted in defects in mitochondrial morphology.<br /><br />Furthermore, the study investigated the relationship between TERC and AMP-activated protein kinase (AMPK), a key regulator of autophagy and ferroptosis. Knockdown of AMPK reduced the expression of autophagy-related proteins, and simultaneous knockdown of TERC and AMPK counteracted the effects of TERC knockdown on autophagy and ferroptosis.<br /><br />Overall, this study suggests that dysregulation of TERC is associated with the development of lung cancer. TERC may play a role in autophagy and ferroptosis through the regulation of AMPK-mediated signaling. Further research is needed to understand the exact mechanisms of TERC function and its clinical significance in lung cancer.

Asset Subtitle

wenmei su

Meta Tag

Speaker wenmei su

Topic Tumor Biology: Preclinical Biology - Molecular Therapeutic Targets

Keywords

lncRNAs

lung adenocarcinoma

TERC

autophagy

ferroptosis

lung cancer

AMPK

RNA sequencing

knockdown

mitochondrial morphology